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BRENDA support

1.4.1.3: glutamate dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about glutamate dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

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L-glutamate
+
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H2O
+
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NAD(P)+
=
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2-oxoglutarate
+
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NH3
+
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NAD(P)H
+
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H+

Synonyms

At5g07440, At5g18170, dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)), dual-coenzyme specific glutamate dehydrogenase, GDH, gdh-1, GDH1, GDH2, GDH3, GdhA, gdhA_1, GDHB, GDHII, GLDH, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2, hGLUD1, hGLUD2, housekeeping glutamate dehydrogenase, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, Legdh1, Membrane protein 50, MP50, NAD(P)+-dependent glutamate dehydrogenase, NAD(P)-dependent GDH, NAD(P)-dependent glutamate dehydrogenase, NAD(P)-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NAD(P)H-utilizing glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, TTC1211, TTC1212, TtGDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                EC 1.4.1.31.4.1.3 glutamate dehydrogenase [NAD(P)+]

Inhibitors

Inhibitors on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+]

for references in articles please use BRENDA:EC1.4.1.3

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17beta-estradiol
-
2-Azido-NAD+
-
0.1 mM, 60% inhibition after 3 min of photolabeling
2-oxobutyrate
-
-
2-oxoglutarate
2-oxovalerate
-
-
4-iodoacetamidosalicylic acid
-
-
5-Bromofuroate
-
-
5-Chlorofuroate
-
-
5-Nitrofuroate
-
-
8-azidoguanosine 5'-triphosphate
-
used for affinity photolabeling, 0.1 mM, 95% inhibition
alanine
-
weak inhibition at pH 8.5, strong inhibition at pH 10.0
AlCl3
-
increase in sensitivity to aluminium as pH decreases, inhibitory effect is predominant below pH 7.0, no effect above pH 8.5. Completely inactivated enzyme contains 2 mol of aluminum per mol of subunit. Citrate, NaF, N-(2-hydroxyethyl) ethylenediaminetriacetic acid or EDTA efficiently protects against inactivation. Citrate and NaF release aluminum from the completely inactivated aluminum-enzyme complex and fully recover enzyme activity. Binding of aluminum induces a decrease in alpha helices and beta sheets and an increase in random coil
alpha-Ketoglutarate oxime
-
-
alpha-Monofluoroglutarate
-
-
alpha-Tetrazole
-
-
Aminooxyacetate
-
5 mM, weak inhibition of isozymes 1-3
AMP
-
inhibits only NADPH-linked activity
cardiolipin
-
-
Chloroquine
-
potent inhibitor of isozymes GDH1 and GDH2 at a dose-dependent manner, the inhibitory effect of chloroquine on GDH2 is abolished by the presence of ADP and L-leucine, whereas GTP does not change the sensitivity to chloroquine inhibition, shows a non-competitive inhibition against 2-oxoglutarate and an uncompetitive inhibition against NADH
citrate
-
10 mM, 60% inhibition of oxidative deamination
corticosterone
-
D-glutamate
dehydroepiandrosterone
-
dehydrotestosterone
-
diethylstilbestrol
Ditetrazole
-
-
fumarate
gamma-Tetrazole
-
-
GDP
strong allosteric inhibitor of GDH1 leading to a 90% reduction of activity. Addition of increasing concentrations of pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm) leads to an increasing protection from GDP inhibition
glutamate
Glutarate
Glyoxal
histidine
-
weak inhibition at pH 8.5, strong inhibition at pH 10.0
imidodiacetic acid
-
-
isophthalate
KCN
-
50 mM, strong inhibition of isozymes 1-3
L-aspartate
-
inhibition of NADPH linked reaction, activation of NAD(H) linked reaction
L-glutamate
lysine
-
weak inhibition at pH 8.5, strong inhibition at pH 10.0
m-bromobenzoate
-
-
m-chlorobenzoate
-
-
m-iodobenzoate
-
-
m-Nitrobenzoate
-
-
malate
-
5 mM, complete inhibition of NADH-linked activity
Methylacetimidate
-
100 mM, moderate inhibition of isozymes 1-3
methylglyoxal
with 1 mM methylglyoxal, GDH activity significantly decreases at 30 min of incubation, and markedly drops by 37% within 5 h compared to control
Mg2+
at 1.0-2.0 mM; at 1.0-2.0 mM
N-(N'-acetyl-4-sulfamoylphenyl)maleimide
-
-
NADP+
-
-
NADPH
-
-
NH4+
-
-
Ni2+
-
1 mM, moderate inhibition of isozymes 1-3
norvaline
-
-
o-phenanthroline
-
5 mM, strong inhibition of isozymes 1-3
o-phthalaldehyde
-
0.1 mM, 98% inhibition after 5 min at 60┬░C, competitive vs. 2-oxoglutarate and NADH
oxaloacetate
oxalylglycine
-
competitive vs. 2-oxoglutarate, uncompetitive vs. NADPH, noncompetitive vs. NH4+
Oxydiglycolic acid
-
-
p-chloromercuribenzoic acid
-
progressive decrease in enzyme activity of both isoenzymes, inhibition is not affected by addition of GTP or ADP
p-hydroxymercuribenzoate
-
5 mM, moderate inhibition of isozymes 1-3
palmitoyl-CoA
Phenylglyoxal
-
4 mM, 75% inhibition, uncompetitive vs. 2-oxoglutarate, noncompetitive vs. NADH
phosphate
-
pH 8.0-9.0: activation, pH 6.0-7.6: almost complete inhibition with 400 mM
phosphatidylserine
-
assumed to be a simple non-competitive inhibition
phosphoenolpyruvate
-
-
pregnenolone
-
progesterone
-
pyridoxal
-
NADH and NADPH protect from inactivation
pyridoxal 5'-phosphate
Sodium acetate
-
at 5┬░C only
sodium dodecylsulfate
-
time-dependent irreversible inhibition, 0.2 mM, 37% inhibition, 0.15 mM, 50% inhibition after 30 min, in the presence of 2-oxoglutarate after 370 min
succinate
-
5 mM, complete inhibition of NADH-linked activity
sulfite
-
uncompetitive
Thiodiglycolic acid
-
-
Zn2+
-
1 mM, strong inhibition of isozymes 1-3
additional information
-