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0.0051 - 14
2-oxoglutarate
0.00294
alpha-ketoglutarate
-
-
0.291
N6-(2-aminoethyl)-NAD+
-
-
0.273
N6-(2-aminoethyl)-NADP+
-
-
0.148
N6-(2-hydroxy-3-trimethylammoniumpropyl)-NAD+
-
-
0.052
N6-(3-sulfonatopropyl)-NAD+
-
-
0.444
poly(ethyleneglycol)-N6-(2-aminoethyl)-NAD+
-
-
-
0.425
poly(ethyleneglycol)-N6-(2-aminoethyl)-NADP+
-
-
-
additional information
additional information
-
0.0051
2-oxoglutarate
recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication
0.0229
2-oxoglutarate
recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication
0.027
2-oxoglutarate
recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication
0.052
2-oxoglutarate
recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication
0.1
2-oxoglutarate
-
euthermic animal, assay at 37°C
0.14
2-oxoglutarate
-
NADP-linked reductive amination
0.18
2-oxoglutarate
-
reductive amination, low-activity form of the enzyme
0.2
2-oxoglutarate
-
cosubstrate NADPH
0.2
2-oxoglutarate
-
reductive amination, high-activity form of the enzyme
0.2
2-oxoglutarate
pH 7.6, 70°C, recombinent enzyme (unheated)
0.25
2-oxoglutarate
-
NADPH-dependent activity
0.25
2-oxoglutarate
-
concentration range: 0.3-4.0 mM
0.25
2-oxoglutarate
pH 7.6, 70°C, native enzyme
0.3
2-oxoglutarate
pH 7.6, 70°C, recombinent enzyme (heated)
0.36
2-oxoglutarate
-
NADH + NH4Cl
0.36
2-oxoglutarate
-
isozyme 1, amination activity, cofactor NADH
0.4
2-oxoglutarate
-
50 mM Tris-HCl, pH 7.2
0.43
2-oxoglutarate
-
hibernating animal, assay at 5°C
0.47
2-oxoglutarate
-
NADPH + NH4Cl
0.53
2-oxoglutarate
-
pH 7.6, 90°C
0.59
2-oxoglutarate
-
isozyme 3, amination activity, cofactor NADH
0.6
2-oxoglutarate
-
cosubstrate NADH
0.62
2-oxoglutarate
-
glutamate dehydrogenase 2, 0.1 mM NADH
0.63
2-oxoglutarate
-
NADPH-dependent amination
0.64
2-oxoglutarate
-
NADPH-dependent amination
0.7
2-oxoglutarate
-
cofactor NADH
0.7
2-oxoglutarate
-
NADPH-dependent amination
0.98
2-oxoglutarate
-
hibernating animal, assay at 37°C
1
2-oxoglutarate
-
liver enzyme
1.1
2-oxoglutarate
-
glutamate dehydrogenase 2, 80 mM NH4+
1.16
2-oxoglutarate
-
mutant C274G, hGDH1
1.2
2-oxoglutarate
-
500 mM Tris-HCl, pH 7.2
1.23
2-oxoglutarate
-
isozyme 2, amination activity, cofactor NADH
1.25
2-oxoglutarate
-
25°C, wild-type enzyme
1.25
2-oxoglutarate
-
pH 8.0, 25°C, wild-type enzyme
1.25
2-oxoglutarate
-
pH 8.0, 25°C, wild-type enzyme hGDH1
1.25
2-oxoglutarate
-
hGDH1, wild-type
1.25
2-oxoglutarate
-
wild-type, hGDH1
1.25
2-oxoglutarate
-
isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C
1.25
2-oxoglutarate
-
wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C
1.27
2-oxoglutarate
-
mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C
1.29
2-oxoglutarate
-
25°C, mutant enzyme K333L
1.29
2-oxoglutarate
-
pH 8.0, 25°C, mutant enzyme Y187E
1.29
2-oxoglutarate
-
mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C
1.31
2-oxoglutarate
-
25°C, mutant enzyme K344L
1.31
2-oxoglutarate
-
25°C, mutant enzyme K346L
1.31
2-oxoglutarate
-
pH 8.0, 25°C, mutant enzyme Y187M
1.34
2-oxoglutarate
-
hGDH1, mutant C323L
1.34
2-oxoglutarate
-
mutant C59G, hGDH1
1.36
2-oxoglutarate
-
hGDH1, mutant C323Y
1.36
2-oxoglutarate
-
mutant C59Y, hGDH1
1.37
2-oxoglutarate
-
mutant C274Y, hGDH1
1.38
2-oxoglutarate
-
hGDH1, mutant C323M
1.39
2-oxoglutarate
-
pH 8.0, 25°C, wild-type enzyme hGDH2
1.39
2-oxoglutarate
-
hGDH1, mutant C323R
1.39
2-oxoglutarate
-
hGDH2, wild-type
1.39
2-oxoglutarate
-
wild-type, hGDH2
1.39
2-oxoglutarate
-
isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C
1.39
2-oxoglutarate
-
wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C
1.4
2-oxoglutarate
-
assay at 37°C
1.4
2-oxoglutarate
-
pH 7.6, 60°C
1.4
2-oxoglutarate
-
hGDH1, mutant C323G
1.4
2-oxoglutarate
-
mutant C274A, hGDH1
1.4
2-oxoglutarate
-
mutant C59A, hGDH1
1.41
2-oxoglutarate
-
pH 8.0, 25°C, mutant enzyme S443R hGDH2
1.41
2-oxoglutarate
-
hGDH2, mutant C323Y
1.44
2-oxoglutarate
-
pH 8.0, 25°C, mutant enzyme Y187R
1.45
2-oxoglutarate
-
25°C, mutant enzyme K337L
1.45
2-oxoglutarate
-
pH 8.0, 25°C, mutant enzyme Y187G
1.45
2-oxoglutarate
-
hGDH2, mutant C323L
1.45
2-oxoglutarate
-
hGDH2, mutant C323M
1.46
2-oxoglutarate
-
pH 8.0, 25°C, mutant enzyme Y187S
1.47
2-oxoglutarate
-
25°C, mutant enzyme S445L
1.5
2-oxoglutarate
-
25°C, mutant enzyme G446D
1.5
2-oxoglutarate
-
hGDH2, mutant C323R
1.5
2-oxoglutarate
-
mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP
1.5 - 2
2-oxoglutarate
-
hGDH2, mutant C323G
1.51
2-oxoglutarate
-
glutamate dehydrogenase 1, 400 mM NH4+
1.51
2-oxoglutarate
-
glutamate dehydrogenase 1, 0.1 mM NADH
1.54
2-oxoglutarate
-
isozyme 1, amination activity, cofactor NADPH
1.58
2-oxoglutarate
-
mutant C274G, hGDH2
1.58
2-oxoglutarate
-
mutant C59Y, hGDH2
1.59
2-oxoglutarate
-
mutant C274A, hGDH2
1.61
2-oxoglutarate
-
mutant C59A, hGDH2
1.66
2-oxoglutarate
-
mutant C274Y, hGDH2
1.67
2-oxoglutarate
-
mutant C59G, hGDH2
1.73
2-oxoglutarate
-
isozyme 3, amination activity, cofactor NADH
1.85
2-oxoglutarate
-
isozyme 2, amination activity, cofactor NADPH
1.86
2-oxoglutarate
-
mutant C119Y, hGDH1
1.9
2-oxoglutarate
-
mutant C119A, hGDH1
1.94
2-oxoglutarate
-
mutant C119G, hGDH1
2
2-oxoglutarate
-
wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP
2.1
2-oxoglutarate
-
cosubstrate NADPH
2.1
2-oxoglutarate
-
glutamate dehydrogenase 1, 40 mM NH4+
2.1
2-oxoglutarate
-
wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP
2.12
2-oxoglutarate
-
mutant C119A, hGDH2
2.18
2-oxoglutarate
-
mutant C119G, hGDH2
2.26
2-oxoglutarate
-
mutant C119Y, hGDH2
2.3
2-oxoglutarate
-
kidney enzyme
2.6
2-oxoglutarate
-
glutamate dehydrogenase 1, 0.2 mM NADPH
3.13
2-oxoglutarate
-
25°C, mutant enzyme H454Y
3.3
2-oxoglutarate
-
cosubstrate NADH
3.5
2-oxoglutarate
-
in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C
3.66
2-oxoglutarate
-
euthermic animal, assay at 5°C
3.7
2-oxoglutarate
-
glutamate dehydrogenase 1, 0.2 mM NADH
3.7
2-oxoglutarate
-
glutamate dehydrogenase 2, 0.2 mM NADPH
4
2-oxoglutarate
-
cofactor NADPH
4.5
2-oxoglutarate
-
cofactor NADH
5
2-oxoglutarate
-
NADH-dependent activity
6.3
2-oxoglutarate
-
cofactor NADPH
7.1
2-oxoglutarate
-
pH 8.0
7.1
2-oxoglutarate
-
NAD-linked reductive amination
10.5
2-oxoglutarate
-
assay at 5°C
14
2-oxoglutarate
-
500 mM Tris-HCl, pH 8.2
0.25
glutamate
-
hibernating animal, assay at 5°C
0.32
glutamate
-
concentration range: 0.05-1.0 mM
0.47
glutamate
-
assay at 5°C
0.5
glutamate
-
euthermic animal, assay at 5°C
2.03
glutamate
-
euthermic animal, assay at 37°C
2.6
glutamate
-
assay at 37°C
4.61
glutamate
-
mitochondrial enzyme, cofactor NAD+
5.2
glutamate
-
hibernating animal, assay at 37°C
5.5
glutamate
-
concentration range: 1.0-10.0 mM
5.93
glutamate
-
enzyme from rough ER, cofactor NAD+
12
glutamate
-
cosubstrate NAD+
20.7
glutamate
-
mitochondrial enzyme, cofactor NADP+
23.8
glutamate
-
enzyme from rough ER, cofactor NADP+
0.24
L-glutamate
-
at pH 8.5
0.27
L-glutamate
recombinant enzyme complex TtGDH-APRTh, low Glu concentration, with AMP, pH and temperature not specified in the publication
0.3
L-glutamate
-
cosubstrate NAD+
0.3
L-glutamate
-
pH 8.0, 70°C
0.4
L-glutamate
-
kidney enzyme
0.45
L-glutamate
recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication
0.6
L-glutamate
-
pH 7.6, 90°C
0.64
L-glutamate
-
isozyme 1, deamination activity
0.77
L-glutamate
recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication
1
L-glutamate
-
1 mM, NAD+
1.01
L-glutamate
-
at pH 7.4
1.1
L-glutamate
-
cosubstrate NADP+
1.2
L-glutamate
recombinant enzyme complex TtGDH-APRTh, low Glu concentration, pH and temperature not specified in the publication
1.6
L-glutamate
-
cofactor NADP+
1.66
L-glutamate
-
cofactor NAD+
1.7
L-glutamate
-
50 mM Tris-HCl, pH 7.2
1.77
L-glutamate
-
pH 9.5, 25°C, mutant enzyme hGDH2D172Y
1.81
L-glutamate
-
pH 9.5, 25°C, wild-type enzyme hGDH2
2.4
L-glutamate
-
NADP-linked oxidative deamination
2.5
L-glutamate
-
pH 7.6, 60°C
2.98
L-glutamate
-
pH 9.5, 25°C, mutant enzyme hGDH2K130Y
3
L-glutamate
-
1 mM, NADP+
3
L-glutamate
-
isozyme 3, deamination activity
3
L-glutamate
-
in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C
3.44
L-glutamate
-
pH 9.5, 25°C, wild-type enzyme
3.44
L-glutamate
-
pH 9.5, 25°C, wild-type enzyme hGDH1
3.52
L-glutamate
-
isozyme 2, deamination activity
3.65
L-glutamate
-
oxidative deamination, low-activity form of the enzyme
3.71
L-glutamate
-
pH 9.5, 25°C, mutant enzyme hGDH1D172Y
3.76
L-glutamate
-
pH 9.5, 25°C, mutant enzyme E279M
3.83
L-glutamate
-
oxidative deamination, high-activity form of the enzyme
3.94
L-glutamate
-
pH 9.5, 25°C, mutant enzyme E279Y
3.98
L-glutamate
-
pH 9.5, 25°C, mutant enzyme E279G
4.05
L-glutamate
-
pH 9.5, 25°C, mutant enzyme E279L
4.12
L-glutamate
-
pH 9.5, 25°C, mutant enzyme E279R
5.51
L-glutamate
-
pH 9.5, 25°C, mutant enzyme hGDH1K130Y
7.3
L-glutamate
-
NAD-linked oxidative deamination
7.99
L-glutamate
-
pH 9.5, 25°C, mutant enzyme hGDH2K94Y
10
L-glutamate
-
NADP+-dependent activity
10.41
L-glutamate
-
pH 9.5, 25°C, mutant enzyme hGDH2K118Y
10.7
L-glutamate
-
wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP
10.8
L-glutamate
-
mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP
12.4
L-glutamate
-
wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP
17.75
L-glutamate
-
pH 9.5, 25°C, mutant enzyme hGDH2G96Y
19
L-glutamate
recombinant enzyme complex TtGDH-APRTh, high Glu concentration, with AMP, pH and temperature not specified in the publication
19.5
L-glutamate
-
500 mM Tris-HCl, pH 9.4
21.82
L-glutamate
-
pH 9.5, 25°C, mutant enzyme hGDH1K94Y
25
L-glutamate
-
0.004 mM, NAD+
25.82
L-glutamate
-
pH 9.5, 25°C, mutant enzyme hGDH1K118Y
30.55
L-glutamate
-
pH 9.5, 25°C, mutant enzyme hGDH1G96Y
31
L-glutamate
-
500 mM Tris-HCl, pH 7.2
52
L-glutamate
recombinant enzyme complex TtGDH-APRTh, high Glu concentration, pH and temperature not specified in the publication
0.014
NAD+
-
-
0.016
NAD+
-
50 mM Tris-HCl, pH 7.2
0.02
NAD+
-
in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C
0.024
NAD+
recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication
0.032
NAD+
-
kidney enzyme
0.056
NAD+
recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication
0.063
NAD+
-
+ L-glutamate
0.1
NAD+
-
pH 8.0, 25°C, recombinant enzyme
0.11
NAD+
-
500 mM Tris-HCl, pH 9.4
0.11
NAD+
-
pH 8.0, 25°C, native enzyme
0.13
NAD+
recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication
0.15
NAD+
-
500 mM Tris-HCl, pH 7.2
0.16
NAD+
recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication
0.2
NAD+
-
isozyme 1, deamination activity
0.26
NAD+
-
isozyme 2, deamination activity
0.32
NAD+
-
isozyme 3, deamination activity
0.364
NAD+
-
mitochondrial enzyme
0.43
NAD+
-
oxidative deamination, low-activity form of the enzyme
0.53
NAD+
-
substrate L-glutamate
0.55
NAD+
-
pH 9.5, 25°C, mutant enzyme hGDH2K94Y
0.58
NAD+
-
pH 9.5, 25°C, mutant enzyme hGDH2G96Y
0.59
NAD+
-
pH 9.5, 25°C, wild-type enzyme hGDH2
0.63
NAD+
-
pH 9.5, 25°C, mutant enzyme hGDH2D172Y
0.65
NAD+
-
pH 9.5, 25°C, mutant enzyme hGDH1G96Y
0.71
NAD+
-
pH 9.5, 25°C, wild-type enzyme
0.71
NAD+
-
pH 9.5, 25°C, wild-type enzyme hGDH1
0.75
NAD+
-
pH 9.5, 25°C, mutant enzyme hGDH2K130Y
0.76
NAD+
-
pH 9.5, 25°C, mutant enzyme hGDH1D172Y
0.79
NAD+
-
pH 9.5, 25°C, mutant enzyme hGDH1K94Y
0.82
NAD+
-
pH 9.5, 25°C, mutant enzyme hGDH2K118Y
0.92
NAD+
-
pH 9.5, 25°C, mutant enzyme hGDH1K130Y
0.924
NAD+
-
enzyme from rough ER
0.94
NAD+
-
pH 9.5, 25°C, mutant enzyme hGDH1K118Y
2.44
NAD+
-
oxidative deamination, high-activity form of the enzyme
3
NAD+
-
NAD-linked oxidative deamination
6.94
NAD+
-
pH 9.5, 25°C, mutant enzyme E279G
7.6
NAD+
-
pH 9.5, 25°C, mutant enzyme E279R
8.35
NAD+
-
pH 9.5, 25°C, mutant enzyme E279Y
9.98
NAD+
-
pH 9.5, 25°C, mutant enzyme E279M
10.01
NAD+
-
pH 9.5, 25°C, mutant enzyme E279L
0.000175
NADH
-
-
0.003
NADH
recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication
0.004
NADH
-
50 mM Tris-HCl, pH 7.2
0.0053
NADH
recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication
0.0058
NADH
recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication
0.007
NADH
-
+ 2-oxoglutarate
0.008
NADH
recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication
0.009
NADH
-
glutamate dehydrogenase 1, 5 mM 2-oxoglutarate
0.009
NADH
-
glutamate dehydrogenase 2, 5 mM 2-oxoglutarate
0.01
NADH
-
500 mM Tris-HCl, pH 8.2
0.012
NADH
-
glutamate dehydrogenase 2, 80 mM NH4+
0.012
NADH
-
500 mM Tris-HCl, pH 7.2
0.015
NADH
-
liver enzyme
0.02
NADH
-
glutamate dehydrogenase 1, 40 mM NH4+
0.022
NADH
-
kidney enzyme
0.04
NADH
-
in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C
0.048
NADH
-
glutamate dehydrogenase 1, 400 mM NH4+
0.05
NADH
-
glutamate dehydrogenase 1, 12.5 mM 2-oxoglutarate
0.07
NADH
-
isozyme 1, amination activity, cofactor NADH
0.075
NADH
-
mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C
0.076
NADH
-
25°C, mutant enzyme K346L
0.079
NADH
-
25°C, mutant enzyme K333L
0.08
NADH
-
pH 8.0, 25°C, wild-type enzyme
0.08
NADH
-
mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C
0.081
NADH
-
25°C, wild-type enzyme
0.081
NADH
-
pH 8.0, 25°C, wild-type enzyme hGDH1
0.081
NADH
-
hGDH1, wild-type
0.081
NADH
-
wild-type, hGDH1
0.081
NADH
-
isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C
0.081
NADH
-
wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C
0.082
NADH
-
pH 8.0, 25°C, mutant enzyme Y187E
0.082
NADH
-
pH 8.0, 25°C, mutant enzyme Y187R
0.083
NADH
-
pH 7.6, 90°C
0.084
NADH
-
pH 8.0, 25°C, mutant enzyme Y187M
0.085
NADH
-
pH 8.0, 25°C, mutant enzyme Y187G
0.086
NADH
-
pH 8.0, 25°C, wild-type enzyme hGDH2
0.086
NADH
-
hGDH2, wild-type
0.086
NADH
-
wild-type, hGDH2
0.086
NADH
-
isozyme GDH2, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C
0.086
NADH
-
wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C
0.088
NADH
-
25°C, mutant enzyme K337L
0.088
NADH
-
pH 8.0, 25°C, mutant enzyme S443R hGDH2
0.088
NADH
-
pH 8.0, 25°C, mutant enzyme Y187S
0.089
NADH
-
25°C, mutant enzyme G446D
0.09
NADH
-
isozyme 3, amination activity, cofactor NADH
0.09
NADH
-
concentration range: 0.04-0.1 mM
0.09
NADH
-
25°C, mutant enzyme K344L
0.09
NADH
-
mutant C119A, hGDH1
0.091
NADH
-
mutant C119Y, hGDH1
0.092
NADH
-
mutant C119G, hGDH1
0.094
NADH
-
mutant C59G, hGDH1
0.097
NADH
-
mutant C59A, hGDH1
0.098
NADH
-
25°C, mutant enzyme S445L
0.098
NADH
-
mutant C119G, hGDH2
0.099
NADH
-
mutant C59A, hGDH2
0.099
NADH
-
mutant C59Y, hGDH1
0.101
NADH
-
mutant C119A, hGDH2
0.102
NADH
-
mutant C59Y, hGDH2
0.106
NADH
-
mutant C59G, hGDH2
0.108
NADH
-
mutant C119Y, hGDH2
0.117
NADH
-
hGDH1, mutant C323L
0.119
NADH
-
hGDH1, mutant C323G
0.119
NADH
-
hGDH1, mutant C323M
0.119
NADH
-
hGDH2, mutant C323M
0.121
NADH
-
hGDH2, mutant C323L
0.122
NADH
-
25°C, mutant enzyme H454Y
0.122
NADH
-
hGDH1, mutant C323R
0.125
NADH
-
hGDH2, mutant C323R
0.128
NADH
-
hGDH2, mutant C323G
0.129
NADH
-
hGDH1, mutant C323Y
0.13
NADH
-
reductive amination, low-activity form of the enzyme
0.138
NADH
-
hGDH2, mutant C323Y
0.14
NADH
-
isozyme 2, amination activity, cofactor NADH
0.169
NADH
-
mutant C274A, hGDH2
0.175
NADH
-
mutant C274G, hGDH2
0.176
NADH
-
mutant C274Y, hGDH2
0.178
NADH
-
mutant C274Y, hGDH1
0.181
NADH
-
mutant C274G, hGDH1
0.189
NADH
-
mutant C274A, hGDH1
0.2
NADH
-
NAD-linked reductive amination
0.5
NADH
-
NADH-dependent activity
0.52
NADH
-
reductive amination, high-activity form of the enzyme
0.98
NADH
-
substrate 2-oxoglutarate
0.004
NADP+
-
NADP+-dependent activity
0.013
NADP+
-
oxidative deamination, low-activity form of the enzyme
0.019
NADP+
-
NADP-linked oxidative deamination
0.025
NADP+
-
+ L-glutamate
0.025
NADP+
-
pH 7.6, 90°C
0.028
NADP+
-
cosubstrate glutamate
0.029
NADP+
-
oxidative deamination, high-activity form of the enzyme
0.035
NADP+
-
in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C
0.08
NADP+
-
concentration range: 0.02-0.2 mM
0.18
NADP+
-
substrate L-glutamate
0.31
NADP+
-
cosubstrate norvaline
0.443
NADP+
-
enzyme from rough ER
0.637
NADP+
-
mitochondrial enzyme
0.006
NADPH
-
NADPH-dependent amination
0.01
NADPH
-
+ 2-oxoglutarate
0.01
NADPH
-
pH 7.6, 60°C
0.011
NADPH
-
reductive amination, low-activity form of the enzyme
0.013
NADPH
-
NADPH-dependent activity
0.013
NADPH
-
NADP-linked reductive amination
0.013
NADPH
-
reductive amination, high-activity form of the enzyme
0.018
NADPH
-
NADPH-dependent amination
0.02
NADPH
-
NADPH-dependent amination
0.028
NADPH
-
glutamate dehydrogenase 1, 10 mM 2-oxoglutarate
0.028
NADPH
-
glutamate dehydrogenase 2, 10 mM 2-oxoglutarate
0.037
NADPH
-
pH 7.6, 90°C
0.04
NADPH
-
concentration range: 0.002-0.1 mM
0.04
NADPH
pH 7.6, 70°C, native enzyme
0.05
NADPH
pH 7.6, 70°C, recombinent enzyme (heated)
0.069
NADPH
-
glutamate dehydrogenase 1, 40 mM NH4+
0.069
NADPH
-
glutamate dehydrogenase 2, 80 mM NH4+
0.1
NADPH
pH 7.6, 70°C, recombinent enzyme (unheated)
0.17
NADPH
-
50 mM Tris-HCl, pH 7.2
0.27
NADPH
-
isozyme 1, amination activity, cofactor NADPH
0.46
NADPH
-
isozyme 3, amination activity, cofactor NADPH
0.54
NADPH
-
500 mM Tris-HCl, pH 7.2
0.56
NADPH
-
substrate 2-oxoglutarate
0.56
NADPH
-
substrate NH3
0.75
NADPH
-
500 mM Tris-HCl, pH 8.2
0.78
NADPH
-
isozyme 2, amination activity, cofactor NADPH
0.38
NH3
-
biphasic Lineweaver-Burk plot suggests 2 Km values
1.25
NH3
-
reductive amination, high-activity form of the enzyme
1.31
NH3
-
reductive amination, low-activity form of the enzyme
1.5
NH3
-
NADPH-dependent amination
1.7
NH3
-
NADP-linked reductive amination
2.4
NH3
-
NADPH-dependent amination
2.5
NH3
pH 7.6, 70°C, recombinent enzyme (unheated)
2.9
NH3
-
NADPH-dependent amination
4.9
NH3
-
NAD-linked reductive amination
5.1
NH3
-
NADP-linked reductive amination
10
NH3
-
in situ activity in 50 mM Tris-HCl buffer and 2 mM EDTA, pH 8, at 37°C
11.4
NH3
pH 7.6, 70°C, recombinent enzyme (heated)
12.8
NH3
-
hGDH1, pH 8.0, temperature not specified in the publication
13
NH3
pH 7.6, 70°C, native enzyme
13.4
NH3
-
wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP
14.7
NH3
-
hGDH2, pH 8.0, temperature not specified in the publication
17.1
NH3
-
wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP
22.2
NH3
-
mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP
29
NH3
recombinant enzyme complex TtGDH-APRTh, pH and temperature not specified in the publication
33
NH3
-
hGDH2, pH 7.5, temperature not specified in the publication
33
NH3
recombinant enzyme complex TtGDH-APRTh, with L-leucine and AMP, pH and temperature not specified in the publication
35
NH3
-
hGDH1, pH 7.5, temperature not specified in the publication
43
NH3
recombinant enzyme complex TtGDH-APRTh, with AMP, pH and temperature not specified in the publication
44
NH3
-
NADPH + 2-oxoglutarate
46
NH3
recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication
50
NH3
-
NADH + 2-oxoglutarate
57.5
NH3
-
hGDH1, pH 7.0, temperature not specified in the publication
62.2
NH3
-
hGDH2, pH 7.0, temperature not specified in the publication
66
NH3
-
NADH-dependent activity
100
NH3
-
biphasic Lineweaver-Burk plot suggests 2 Km values
0.0424
NH4+
-
-
1.3
NH4+
-
concentration range: 0.2-5 mM
5.8
NH4+
-
glutamate dehydrogenase 1, 0.2 mM NADPH
7
NH4+
-
glutamate dehydrogenase 1, 0.2 mM NADPH
7
NH4+
-
euthermic animal, assay at 37°C
10.4
NH4+
-
glutamate dehydrogenase 1, 10 mM 2-oxoglutarate
12.1
NH4+
-
hibernating animal, assay at 5°C
12.9
NH4+
-
glutamate dehydrogenase 2, 5 mM 2-oxoglutarate
13
NH4+
-
isozyme 2, amination activity, cofactor NADH
13
NH4+
-
concentration range: 10-200 mM
15
NH4+
-
cosubstrates NADPH + 2-oxoglutarate
15.8
NH4+
-
glutamate dehydrogenase 1, 5 mM 2-oxoglutarate
15.8
NH4+
-
hibernating animal, assay at 37°C
17.8
NH4+
-
assay at 37°C
19
NH4+
-
glutamate dehydrogenase 1, 10 mM 2-oxoglutarate
21.7
NH4+
-
glutamate dehydrogenase 1, 0.1 mM NADH
22.8
NH4+
-
glutamate dehydrogenase 1, 0.1 mM NADH
24.7
NH4+
-
euthermic animal, assay at 5°C
27
NH4+
-
cosubstrates NADH + 2-oxoglutarate
30
NH4+
-
isozyme 1, amination activity, cofactor NADH
36
NH4+
-
isozyme 1, amination activity, cofactor NADPH
41
NH4+
-
isozyme 3, amination activity, cofactor NADPH
44
NH4+
-
isozyme 3, amination activity, cofactor NADH
53
NH4+
-
isozyme 2, amination activity, cofactor NADPH
58
NH4+
-
500 mM Tris-HCl, pH 8.2
77
NH4+
-
500 mM Tris-HCl, pH 7.2
106
NH4+
-
glutamate dehydrogenase 1, 12.5 mM 2-oxoglutarate
115.1
NH4+
-
glutamate dehydrogenase 1, 0.2 mM NADH
160
NH4+
-
50 mM Tris-HCl, pH 7.2
additional information
additional information
-
-
-
additional information
additional information
-
Km-values for 2-oxoglutaratefor mutant enzyme R443S, recombinant wild-type enzyme and wild-type enzyme from human liver
-
additional information
additional information
kinetic analysis in wild-type and transgenic overexpressing brain cell fractions, overview
-
additional information
additional information
-
kinetic analysis in wild-type and transgenic overexpressing brain cell fractions, overview
-
additional information
additional information
-
lowering the pH of the buffer from pH 8.0 to pH 7.0 increases the Km for ammonia substantially, i.e. for hGDH1 from 12.8 mM to 57.5 mM, and for hGDH2: from 14.7 mM to 62.2 mM, thus essentially precluding reductive amination
-
additional information
additional information
when assayed in triethanolamine buffer, pH 8.0, at 1.0 mM ADP, hGDH1 and hGDH2 show similar catalytic properties (Vmax and Kms for 2-oxoglutarate, ammonia and glutamate), kinetics, overview
-
additional information
additional information
when assayed in triethanolamine buffer, pH 8.0, at 1.0 mM ADP, hGDH1 and hGDH2 show similar catalytic properties (Vmax and Kms for 2-oxoglutarate, ammonia and glutamate), kinetics, overview
-
additional information
additional information
-
when assayed in triethanolamine buffer, pH 8.0, at 1.0 mM ADP, hGDH1 and hGDH2 show similar catalytic properties (Vmax and Kms for 2-oxoglutarate, ammonia and glutamate), kinetics, overview
-
1.4.1.3 glutamate dehydrogenase [NAD(P)+]
results (
results (
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