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Literature summary for 1.4.1.3 extracted from

  • Choi, M.M.; Hwang, E.Y.; Kim, E.A.; Huh, J.W.; Cho, S.W.
    Identification of amino acid residues responsible for different GTP preferences of human glutamate dehydrogenase isozymes (2008), Biochem. Biophys. Res. Commun., 368, 742-747.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
chimeric mutant enzymes expressed in Escherichia coli DE3 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
L415M/S443R/A456G triple mutant hGDH2(hGDH1390-465)hGDH2 (amino acid segment 390-465 of hGDH2 replaced by the corresponding hGDH1 segment) Homo sapiens
M415L/R443S/G456A triple mutant hGDH1(hGDH2390-465)hGDH1 (amino acid segment 390-465 of hGDH1 replaced by the corresponding hGDH2 segment) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GTP
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
NADH mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens
0.08
-
NADH mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens
0.081
-
NADH wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens
0.086
-
NADH wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens
1.25
-
2-oxoglutarate wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens
1.27
-
2-oxoglutarate mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens
1.29
-
2-oxoglutarate mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens
1.39
-
2-oxoglutarate wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2',5'-ADP Sepharose 4B column and FPLC Resource-Q column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
Homo sapiens 2-oxoglutarate + NH3 + NADH + H+
-
?
L-glutamate + H2O + NADP+
-
Homo sapiens 2-oxoglutarate + NH3 + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
GDH1 isozyme Homo sapiens
GDH2 isozyme Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
101
-
NADH mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens
104
-
NADH wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens
118
-
NADH mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens
130
-
NADH wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25┬░C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.001
-
isozyme GDH1 Homo sapiens GTP
0.07
-
isozyme GDH2 Homo sapiens GTP