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Literature summary for 1.4.1.3 extracted from
- Properties of glutamate dehydrogenase from Lemna minor (1976), Phytochemistry, 15, 1611-1617.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | 1 mM, 65% inhibition of NADH reaction due to chelating properties of ATP | Lemna minor |  |
| EDTA | complete loss of NADH and NAD+ activities, NADPH activity unaffected | Lemna minor | |
| GTP | 1 mM, 42% inhibition due to chelating properties of GTP | Lemna minor | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
NADH | - |
Lemna minor | |
| 0.13 | - |
NADPH | - |
Lemna minor | |
| 0.46 | - |
NAD+ | - |
Lemna minor | |
| 2.1 | - |
2-oxoglutarate | cosubstrate NADPH | Lemna minor | |
| 3.3 | - |
2-oxoglutarate | cosubstrate NADH | Lemna minor | |
| 12 | - |
glutamate | cosubstrate NAD+ | Lemna minor | |
| 15 | - |
NH4+ | cosubstrates NADPH + 2-oxoglutarate | Lemna minor | |
| 27 | - |
NH4+ | cosubstrates NADH + 2-oxoglutarate | Lemna minor | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Lemna minor | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lemna minor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lemna minor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.36 | - |
cofactor NADPH | Lemna minor |
| 3.5 | - |
cofactor NADH | Lemna minor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH3 + NADH + H+ | - |
Lemna minor | L-glutamate + H2O + NAD+ | - |
r | |
| L-glutamate + H2O + NAD(P)+ | - |
Lemna minor | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
| L-glutamate + H2O + NAD+ | - |
Lemna minor | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
short periods | Lemna minor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
NADPH + 2-oxoglutarate + NH3 | Lemna minor |
| 8.2 | - |
NADH + NH3 + 2-oxoglutarate | Lemna minor |
| 8.8 | - |
NAD+ + glutamate | Lemna minor |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Lemna minor | |
| NADH | - |
Lemna minor | |
| NADPH | - |
Lemna minor | |
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