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Literature summary for 1.4.1.3 extracted from

  • Veronese, F.M.; Bevilacqua, R.; Boccu, E.; Brown, D.M.
    Purification, characteristics and sequence of a peptide containing an essential lysine residue (1976), Biochim. Biophys. Acta, 445, 1-13.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP stimulation Thunnus thynnus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Thunnus thynnus

Inhibitors

Inhibitors Comment Organism Structure
GTP
-
Thunnus thynnus
isophthalate competitive vs. glutamate Thunnus thynnus
pyridoxal 5'-phosphate 0.11 mM, approx. 30% inactivation after 10 min, 0.78 mM, 80% inactivation, inactivation is completely reversed by dialysis Thunnus thynnus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53900
-
6 * 53900, sedimentation equilibrium of enzyme treated with 6 M guanidinium and 0.5% 2-mercaptoethanol Thunnus thynnus
333000
-
-
Thunnus thynnus

Organism

Organism UniProt Comment Textmining
Thunnus thynnus
-
tuna
-

Purification (Commentary)

Purification (Comment) Organism
-
Thunnus thynnus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Thunnus thynnus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
1.93
-
after 3rd crystallization Thunnus thynnus

Storage Stability

Storage Stability Organism
suspended in ammonium sulfate, stable for months Thunnus thynnus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
-
Thunnus thynnus 2-oxoglutarate + NH3 + NAD(P)H
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 53900, sedimentation equilibrium of enzyme treated with 6 M guanidinium and 0.5% 2-mercaptoethanol Thunnus thynnus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25
-
glutamate oxidation Thunnus thynnus
510
-
2-oxoglutarate reduction Thunnus thynnus

Cofactor

Cofactor Comment Organism Structure
NADH reaction rate NADH/NADPH: 15 Thunnus thynnus
NADPH reaction rate NADH/NADPH: 15 Thunnus thynnus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.28
-
isophthalate
-
Thunnus thynnus