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Literature summary for 1.4.1.3 extracted from

  • Kimura, K.; Miyakawa, A.; Imai, T.; Sasakawa, T.
    Glutamate dehydrogenase from Bacillus subtilis PCI 219. I. Purification and properties (1977), J. Biochem., 81, 467-476.
    View publication on PubMed

General Stability

General Stability Organism
freeze-thawing decreases activity Bacillus subtilis
glycerol, 5-20% v/v, stabilizes Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
citrate 10 mM, 60% inhibition of oxidative deamination Bacillus subtilis
oxaloacetate 5 mM, 79% inhibition of oxidative deamination Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
x * 57000, SDS-PAGE Bacillus subtilis
270000
-
gel filtration, sucrose density gradient centrifugation Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis PCI 219
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
47
-
-
Bacillus subtilis

Storage Stability

Storage Stability Organism
0┬░C, 50 mM Na,K phosphate buffer, pH 5.5, stable Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
-
Bacillus subtilis 2-oxoglutarate + NH3 + NAD(P)H
-
r
L-glutamate + H2O + NAD(P)+
-
Bacillus subtilis PCI 219 2-oxoglutarate + NH3 + NAD(P)H
-
r

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Bacillus subtilis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
66
-
10 min, 50% loss of activity Bacillus subtilis
74
-
10 min, complete loss of activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
NADPH + NH3 + 2-oxoglutarate Bacillus subtilis
7.6
-
NADP+ + glutamate Bacillus subtilis
7.8
-
NADH + NH3 + 2-oxoglutarate Bacillus subtilis
8.2
-
NAD+ + H2O + glutamate Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NADPH NADPH/NADH ratio of reaction rate: 10/1, NAD+/NADH ratio of reaction rate: 5/1 Bacillus subtilis