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Literature summary for 1.4.1.3 extracted from

  • Islam, M.M.; Nautiyal, M.; Wynn, R.M.; Mobley, J.A.; Chuang, D.T.; Hutson, S.M.
    The branched chain amino acid (BCAA) metabolon: Interaction of glutamate dehydrogenase with the mitochondrial branched chain aminotransferase (BCATm) (2010), J. Biol. Chem., 285, 265-276.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ADP ADP induces an allosteric conformational change in GDH1, leading to a 2fold enhanced oxidative deamination Rattus norvegicus
leucine enhances the oxidative deamination reaction of GDH1 Rattus norvegicus
PMP-BCATm pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm) accelerates the oxidative deamination reaction of GDH1in the presence of branched-chain amino acids (Leu, Ile, Val). Reductive amination reaction is not affected Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
GDP strong allosteric inhibitor of GDH1 leading to a 90% reduction of activity. Addition of increasing concentrations of pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm) leads to an increasing protection from GDP inhibition Rattus norvegicus
GTP
-
Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P10860
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
pancreas
-
Rattus norvegicus
-
skeletal muscle
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + NAD+ + H2O
-
Rattus norvegicus 2-oxoglutarate + NADH + NH3
-
r

Subunits

Subunits Comment Organism
homohexamer method of determination not further specified Rattus norvegicus

Synonyms

Synonyms Comment Organism
GDH1
-
Rattus norvegicus
glutamate dehydrogenase
-
Rattus norvegicus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reductive amination at Rattus norvegicus
8
-
oxidative deamination at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+ GDH1 uses NAD+ or NADP+ as coenzyme Rattus norvegicus
NADP+ GDH1 uses NAD+ or NADP+ as coenzyme Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00005
-
GTP GTP alone Rattus norvegicus
0.00156
-
GTP molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 1 Rattus norvegicus
0.00312
-
GTP molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 2 Rattus norvegicus
0.00795
-
GTP molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 6 Rattus norvegicus
0.00806
-
GTP molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 4 Rattus norvegicus

General Information

General Information Comment Organism
metabolism GDH1 is the protein partner for pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm). Facilitating the recycling of BCATm to form metabolon, GDH1 acts as a catalytic machine Rattus norvegicus