Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.4.1.3: glutamate dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about glutamate dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

Show molfile
L-glutamate
+
Show molfile
H2O
+
Show molfile
NAD(P)+
=
Show molfile
2-oxoglutarate
+
Show molfile
NH3
+
Show molfile
NAD(P)H
+
Show molfile
H+

Synonyms

At5g07440, At5g18170, dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)), dual-coenzyme specific glutamate dehydrogenase, GDH, gdh-1, GDH1, GDH2, GDH3, GdhA, gdhA_1, GDHB, GDHII, GLDH, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2, hGLUD1, hGLUD2, housekeeping glutamate dehydrogenase, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, Legdh1, Membrane protein 50, MP50, NAD(P)+-dependent glutamate dehydrogenase, NAD(P)-dependent GDH, NAD(P)-dependent glutamate dehydrogenase, NAD(P)-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NAD(P)H-utilizing glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, TTC1211, TTC1212, TtGDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                EC 1.4.1.31.4.1.3 glutamate dehydrogenase [NAD(P)+]

Molecular Weight

Molecular Weight on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+]

for references in articles please use BRENDA:EC1.4.1.3

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
160000
-
mutant enzyme H454Y, gel filtration
210000
-
gel filtration
240000 - 260000
-
sucrose density gradient sedimentation
250000
-
liver enzyme, sedimentation velocity, light scattering
250500
-
gel filtration
266000 - 269000
-
gel filtration
270000
284000
285000
-
gel filtration
290000
300000 - 350000
315000
recombinant His-tagged binary complex TtGDH, gel filtration
320000
-
enzyme from hibernating animals, gel filtration
330000
331000
333000
335000
-
enzyme from euthermic animals, gel filtration
336000
-
SDS-PAGE
340000
40500
-
6 * 40500, SDS-PAGE
42000
-
SDS-PAGE
44000
45000
46000
460000
-
gel filtration
46078
x * 46078, calculated from sequence
47040
6 * 47040, calculated from sequence, the natural enzyme was purified only as a hexameric form, whereas the recombinant enzyme was purified as both monomeric and hexameric forms. Only the enzyme in a hexameric form is active. Upon heat treatment (70┬░C for 15 min), the inactive monomeric form of the recombinant enzyme is at least partially associated with the hexameric form
47122
x * 47122, calculated from sequence
47300
48000
49000
-
6 * 49000, SDS-PAGE
50000
-
6 * 50000, membrane-bound liver enzyme, SDS-PAGE
52000
-
4 * 52000, SDS-PAGE
53900
-
6 * 53900, sedimentation equilibrium of enzyme treated with 6 M guanidinium and 0.5% 2-mercaptoethanol
54000
-
6 * 54000, SDS-PAGE
56000
56500
57000
57500
-
x * 57500, recombinant enzyme, SDS-PAGE
59500
-
6 * 59500, enzyme from both euthermic and hibernating animals
60000
-
native gradient polyacryamide gel electrophoresis, 6 * 60000 Da
61000
-
8 * 61000, or aggregate of two tetramers, SDS-PAGE
61400
gel filtration