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BRENDA support

1.4.1.3: glutamate dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about glutamate dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

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L-glutamate
+
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H2O
+
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NAD(P)+
=
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2-oxoglutarate
+
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NH3
+
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NAD(P)H
+
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H+

Synonyms

At5g07440, At5g18170, dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)), dual-coenzyme specific glutamate dehydrogenase, GDH, gdh-1, GDH1, GDH2, GDH3, GdhA, gdhA_1, GDHB, GDHII, GLDH, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2, hGLUD1, hGLUD2, housekeeping glutamate dehydrogenase, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, Legdh1, Membrane protein 50, MP50, NAD(P)+-dependent glutamate dehydrogenase, NAD(P)-dependent GDH, NAD(P)-dependent glutamate dehydrogenase, NAD(P)-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NAD(P)H-utilizing glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, TTC1211, TTC1212, TtGDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                EC 1.4.1.31.4.1.3 glutamate dehydrogenase [NAD(P)+]

Purification

Purification on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+]

for references in articles please use BRENDA:EC1.4.1.3

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2',5'-ADP Sepharose 4B column and FPLC Resource-Q column chromatography
-
2,5-ADP-Sepharose column chromatography and Resource-Q column chromatography
-
affinity-purified
-
ammonium sulfate fractionation, phenyl-Sepharose column chromatography, and hydroxyapatite chromatography
-
ammonium sulfate, DEAE-cellulose, affinity precipitation with adipo-N2,N2-dihydrazido-bis(N6-carboxymethyl-NAD+)
-
ammonium sulfate, phenyl Sepharose, Superdex 200, Q Sepharose
-
brain isoenzymes 1 and 2
-
by gel filtration
-
DEAE-Sepharose, ATP-agarose, Resource Q
-
enzyme from euthermic and hibernating animals
-
heat denaturation at 70┬░C and Ni-agarose affinity column chromatography
-
HitrapQ, Hitrap blue, recombinant and native enzyme
-
hydroxylapatite, DEAE-Toyopearl, GTP-Sepharose
-
isoenzymes, glutamate dehydrogenases 1, 2 and 3
-
native and recombinant enzyme
-
native enzyme 22.7fold by anion exchange chromatography and gel filtration to homogeneity
-
partial
recombinant His-tagged ternary complex TtGDH-APRTh from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant GDH2 from Escherichia coli
-
recombinant His-tagged wild-type and mutant GDHA and GDHB from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by affinity chromatography and gel filtration, and of untagged proteins by heat treatment at 90┬░C for 20 min, hydrophobic interaction and anion exchange chromatography, and gel filtration to homogeneity
-
recombinant isozyme hGDH1 mutants from Spodoptera frugiperda SF21 cells by ammonium sulfate fractionation, hydrophobic interaction chromatography, and hydroxyapatite chromatography
recombinant isozyme hGDH2 from Spodoptera frugiperda SF21 cells by ammonium sulfate fractionation, hydrophobic interaction chromatography, and hydroxyapatite chromatography
recombinant protein
recombinant wild-type GLUD1 and GLUD2 from Spodoptera frugiperda Sf21 cells by ammonim sulfate fractionation, and hydrophobic interaction and hydroxyapatite chromatography
-
to homogeneity
-
using ammonium sulphate fractionation, hydrophobic interaction (using a phenylsepharose high performance column) and hydroxyapatite chromatography
-
wild-type and Glu279 mutant enzymes
-
wild-type and mutant enzyme, Q-Sepharose, omega-aminopentyl column, GTP-agarose
-