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Literature summary for 1.4.1.3 extracted from

  • Engelhardt, H.; Klemme, J.H.
    Characterization of an allosteric, nucleotide-unspecific glutamate dehydrogenase from Rhodopseudomonas sphaeroides (1978), FEMS Microbiol. Lett., 3, 287-290.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
AMP activates NADH-linked glutamate synthesis, inhibits NADPH-linked activity Cereibacter sphaeroides
AMP activation only in biosynthetic direction Cereibacter sphaeroides
L-aspartate 2fold activation of glutamate synthesis Cereibacter sphaeroides

General Stability

General Stability Organism
AMP stabilizes Cereibacter sphaeroides
L-aspartate stabilizes Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
AMP inhibits only NADPH-linked activity Cereibacter sphaeroides
ATP
-
Cereibacter sphaeroides
fumarate
-
Cereibacter sphaeroides
L-aspartate inhibition of NADPH linked reaction, activation of NAD(H) linked reaction Cereibacter sphaeroides
malate 5 mM, complete inhibition of NADH-linked activity Cereibacter sphaeroides
phosphoenolpyruvate
-
Cereibacter sphaeroides
succinate 5 mM, complete inhibition of NADH-linked activity Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
NADH 50 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides
0.01
-
NADH 500 mM Tris-HCl, pH 8.2 Cereibacter sphaeroides
0.012
-
NADH 500 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides
0.016
-
NAD+ 50 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides
0.11
-
NAD+ 500 mM Tris-HCl, pH 9.4 Cereibacter sphaeroides
0.15
-
NAD+ 500 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides
0.17
-
NADPH 50 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides
0.4
-
2-oxoglutarate 50 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides
0.54
-
NADPH 500 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides
0.75
-
NADPH 500 mM Tris-HCl, pH 8.2 Cereibacter sphaeroides
1.2
-
2-oxoglutarate 500 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides
1.7
-
L-glutamate 50 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides
14
-
2-oxoglutarate 500 mM Tris-HCl, pH 8.2 Cereibacter sphaeroides
19.5
-
L-glutamate 500 mM Tris-HCl, pH 9.4 Cereibacter sphaeroides
31
-
L-glutamate 500 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides
58
-
NH4+ 500 mM Tris-HCl, pH 8.2 Cereibacter sphaeroides
77
-
NH4+ 500 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides
160
-
NH4+ 50 mM Tris-HCl, pH 7.2 Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
strain DSM 158
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
4
-
cofactor NADPH Cereibacter sphaeroides
13
-
cofactor NADH Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
-18Ā°C, several months, no loss of activity Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+
-
Cereibacter sphaeroides L-glutamate + H2O + NAD+
-
r
L-glutamate + H2O + NAD(P)+
-
Cereibacter sphaeroides 2-oxoglutarate + NH3 + NAD(P)H
-
r
L-glutamate + H2O + NAD+
-
Cereibacter sphaeroides 2-oxoglutarate + NH3 + NADH + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
NADH + NH3 + 2-oxoglutarate Cereibacter sphaeroides
9.3
-
NAD+ + glutamate Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Cereibacter sphaeroides
NADH
-
Cereibacter sphaeroides
NADP+ only 5% of the activity with NAD+ Cereibacter sphaeroides
NADPH
-
Cereibacter sphaeroides