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1.4.1.3: glutamate dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about glutamate dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

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L-glutamate
+
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H2O
+
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NAD(P)+
=
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2-oxoglutarate
+
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NH3
+
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NAD(P)H
+
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H+

Synonyms

At5g07440, At5g18170, dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)), dual-coenzyme specific glutamate dehydrogenase, GDH, gdh-1, GDH1, GDH2, GDH3, GdhA, gdhA_1, GDHB, GDHII, GLDH, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2, hGLUD1, hGLUD2, housekeeping glutamate dehydrogenase, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, Legdh1, Membrane protein 50, MP50, NAD(P)+-dependent glutamate dehydrogenase, NAD(P)-dependent GDH, NAD(P)-dependent glutamate dehydrogenase, NAD(P)-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NAD(P)H-utilizing glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, TTC1211, TTC1212, TtGDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                EC 1.4.1.31.4.1.3 glutamate dehydrogenase [NAD(P)+]

General Stability

General Stability on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+]

for references in articles please use BRENDA:EC1.4.1.3

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol stabilizes
-
absolutely stable at 4┬░C or at room temperature for at least 6 months
-
AMP stabilizes
-
freeze-thawing decreases activity
-
glutathione stabilizes
-
glycerol, 20% v/v, protects against cold inactivation
-
glycerol, 5-20% v/v, stabilizes
-
glyoxyl agarose-GDH immobilization is more stable than CNBr-agarose preparations or than the soluble enzyme, glyoxyl agarose derivative, prepared at 4┬░C for 15 min, immobilizes around 50% of the enzyme, but the enzyme retains almost full activity after the immobilization
-
guanidine-dependent inactivation of the enzyme at 20┬░C is irreversible above 1.5 M guanidine hydrochloride
-
L-aspartate stabilizes
-
SDS: 0.1% v/v, no loss of activity after 12 h, 0.5%, half-life 5 h
-
strong dependence on high salt concentrations for stability
-
urea: 4 M, 10% loss of activity after 12 h, 7.5 M, half-life 9 h
-