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Literature summary for extracted from

  • Mc Kenzie, E.A.; Lees, E.M.
    Glutamate dehydrogenase activity in developing soybean seed: isolation and characterization of three forms of the enzyme (1981), Arch. Biochem. Biophys., 212, 290-297.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 2fold activation Glycine max
glutathione 2fold activation Glycine max

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Glycine max
glutathione stabilizes Glycine max


Localization Comment Organism GeneOntology No. Textmining
Glycine max 9507

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000 350000 isoenzymes GDH1, GDH2, disc gel electrophoresis Glycine max


Organism UniProt Comment Textmining
Glycine max
glutamate dehydrogenase 1 and 2

Source Tissue

Source Tissue Comment Organism Textmining
Glycine max
seed glutamate dehydrogenase 1 is found only in the developing seed Glycine max

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
glutamate dehydrogenase 2 Glycine max
glutamate dehydrogenase 1 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
Glycine max 2-oxoglutarate + NH3 + NAD(P)H

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
NADPH Glycine max
Glycine max
2-oxoglutarate + NH3 + NAD(P)H Glycine max
NADP+ + glutamate Glycine max
NAD+ + glutamate Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.8 NAD(P)H + 2-oxoglutarate + NH3, more than 50% of activity maximum at pH 7.5 and 8.8 Glycine max
8 9.2 deamination, more than 50% of activity maximum at pH 8.0 and 9.2 Glycine max


Cofactor Comment Organism Structure
Glycine max
NADH reaction rate NADH/NADPH: 4.5 (GDH2) Glycine max
NADH reaction rate NADH/NADPH: 1.3 (GDH1) Glycine max
Glycine max
NADPH glutamate dehydrogenase 2, reaction rate NADH/NADPH: 4.4 Glycine max
NADPH glutamate dehydrogenase 1, reaction rate NADH/NADPH: 1.3 Glycine max