Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.3 extracted from

  • Mc Kenzie, E.A.; Lees, E.M.
    Glutamate dehydrogenase activity in developing soybean seed: isolation and characterization of three forms of the enzyme (1981), Arch. Biochem. Biophys., 212, 290-297.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 2fold activation Glycine max
glutathione 2fold activation Glycine max

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Glycine max
glutathione stabilizes Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Glycine max 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000 350000 isoenzymes GDH1, GDH2, disc gel electrophoresis Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
glutamate dehydrogenase 1 and 2
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Glycine max
-
seed glutamate dehydrogenase 1 is found only in the developing seed Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
1.2
-
glutamate dehydrogenase 2 Glycine max
1.8
-
glutamate dehydrogenase 1 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
-
Glycine max 2-oxoglutarate + NH3 + NAD(P)H
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
NADPH Glycine max
8
-
-
Glycine max
8.3
-
2-oxoglutarate + NH3 + NAD(P)H Glycine max
8.7
-
NADP+ + glutamate Glycine max
8.9
-
NAD+ + glutamate Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.8 NAD(P)H + 2-oxoglutarate + NH3, more than 50% of activity maximum at pH 7.5 and 8.8 Glycine max
8 9.2 deamination, more than 50% of activity maximum at pH 8.0 and 9.2 Glycine max

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Glycine max
NADH reaction rate NADH/NADPH: 4.5 (GDH2) Glycine max
NADH reaction rate NADH/NADPH: 1.3 (GDH1) Glycine max
NADP+
-
Glycine max
NADPH glutamate dehydrogenase 2, reaction rate NADH/NADPH: 4.4 Glycine max
NADPH glutamate dehydrogenase 1, reaction rate NADH/NADPH: 1.3 Glycine max