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Literature summary for 1.4.1.3 extracted from

  • Consalvi, V.; Chiaraluce, R.; Politi, L.; Pasquo, A.; De Rosa, M.; Scandurra, R.
    Glutamate dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus: studies on thermal and guanidine-dependent inactivation. (1993), Biochim. Biophys. Acta, 1202, 207-215.
    View publication on PubMed

General Stability

General Stability Organism
guanidine-dependent inactivation of the enzyme at 20┬░C is irreversible above 1.5 M guanidine hydrochloride Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADPH + NH3
-
Saccharolobus solfataricus L-glutamate + NADP+ + H2O
-
r

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
NAD(P)-dependent glutamate dehydrogenase
-
Saccharolobus solfataricus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
90
-
incubation for 1 h at 0.2 mg/ml results in 90% loss of activity, incubation for 24 h at 0.8 mg/ml results in 30% loss of activity. Inactivation is irreversible. 3 M guanidine-HCl increases the half-life of the enzyme at 90┬░C and 0.2 mg/ml 6fold. Half-life at 90┬░C and 0.2 mg/ml protein concentration increases more than 6fold in the presence of 0.4 M Na2SO4 and decreases 4fold in the presence of 0.4 M NaSCN Saccharolobus solfataricus
95
-
half-life: 15 min at 0.4 mg/ml, 30 min at 0.8 mg/ml. Temperature-dependent inactivation of the enzyme is irreversible, this process is accompanied by a progressive increase in hydrophobic surface area which leads to protein precipitation Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2 6.8 maximal stablility Saccharolobus solfataricus