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Literature summary for 1.4.1.3 extracted from

  • Bonete, M.J.; Perez-Pomares, F.; Diaz, S.; Ferrer, J.; Oren, A.
    Occurrence of two different glutamate dehydrogenase activities in the halophilic bacterium Salinibacter ruber (2003), FEMS Microbiol. Lett., 226, 181-186.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP activates in presence of 3 M NaCl or 3 M KCl or in absence of salts Salinibacter ruber
ATP activates in presence of 3 M NaCl or in absence of salts, slight inhibition in presence of 3 M KCl Salinibacter ruber
L-His activates in presence of 3 M NaCl, 3 M Kcl or in absence of salts Salinibacter ruber
L-Leu activates in presence of 3 M NaCl, 3 M Kcl or in absence of salts Salinibacter ruber

General Stability

General Stability Organism
strong dependence on high salt concentrations for stability Salinibacter ruber

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.064
-
NADH pH 8.0 Salinibacter ruber
0.42
-
NADPH pH 8.0 Salinibacter ruber
7.1
-
2-oxoglutarate pH 8.0 Salinibacter ruber
68
-
NH3 pH 8.0 Salinibacter ruber

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Salinibacter ruber 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 3 mM, activates to 144% of control Salinibacter ruber
NaCl 3 M, 67fold activation Salinibacter ruber

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
8 * 61000, or aggregate of two tetramers, SDS-PAGE Salinibacter ruber
460000
-
gel filtration Salinibacter ruber

Organism

Organism UniProt Comment Textmining
Salinibacter ruber
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Salinibacter ruber

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
additional information
-
-
Salinibacter ruber

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH
-
Salinibacter ruber L-glutamate + H2O + NAD+
-
r
2-oxoglutarate + NH3 + NADPH + H+
-
Salinibacter ruber L-glutamate + H2O + NADP+
-
r

Subunits

Subunits Comment Organism
octamer 8 * 61000, or aggregate of two tetramers, SDS-PAGE Salinibacter ruber

Synonyms

Synonyms Comment Organism
GDHII
-
Salinibacter ruber

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
amination reaction Salinibacter ruber
8.5
-
deamination, in 50 mM Tris-HCl buffer containing 3 M KCl Salinibacter ruber
9.5
-
deamination, in 50 mM CHES buffer (N-cyclohexyl-2-aminoethanesulfonic acid) containing 3 M NaCl Salinibacter ruber

Cofactor

Cofactor Comment Organism Structure
NADH
-
Salinibacter ruber
NADPH at lower maximal rate than with NADH Salinibacter ruber