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Literature summary for 1.4.1.3 extracted from

  • Schinkinger, M.F.; Redl, B.; St÷ffler, G.
    Purification and properties of an extreme thermostable glutamate dehydrogenase from the archaebacterium Sulfolobus solfataricus (1991), Biochim. Biophys. Acta, 1073, 142-148.
    View publication on PubMed

General Stability

General Stability Organism
SDS: 0.1% v/v, no loss of activity after 12 h, 0.5%, half-life 5 h Saccharolobus solfataricus
urea: 4 M, 10% loss of activity after 12 h, 7.5 M, half-life 9 h Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
D-glutamate 2 mM, 11% inhibition, 6 mM, 34% inhibition Saccharolobus solfataricus
pyridoxal 5'-phosphate 2 mM, complete loss of activity Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
NADH + 2-oxoglutarate Saccharolobus solfataricus
0.01
-
NADPH + 2-oxoglutarate Saccharolobus solfataricus
0.025
-
NADP+ + L-glutamate Saccharolobus solfataricus
0.063
-
NAD+ + L-glutamate Saccharolobus solfataricus
0.2
-
2-oxoglutarate cosubstrate NADPH Saccharolobus solfataricus
0.3
-
L-glutamate cosubstrate NAD+ Saccharolobus solfataricus
0.6
-
2-oxoglutarate cosubstrate NADH Saccharolobus solfataricus
1.1
-
L-glutamate cosubstrate NADP+ Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
6 * 44000, SDS-PAGE Saccharolobus solfataricus
270000
-
gel filtration Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 50% v/v, complete loss of activity Saccharolobus solfataricus
isopropanol 50% v/v, complete loss of activity Saccharolobus solfataricus
Methanol 50% v/v, complete loss of activity Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
423
-
at 70┬░C Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
-70┬░C, stable for 6 months Saccharolobus solfataricus
4┬░C, 20% loss of activity after 40 days Saccharolobus solfataricus
4┬░C, more than 20 days, no loss of activity Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH
-
Saccharolobus solfataricus L-glutamate + H2O + NAD+
-
?
L-glutamate + H2O + NAD(P)+ highly specific for 2-oxoglutarate and glutamate Saccharolobus solfataricus 2-oxoglutarate + NH3 + NAD(P)H
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 44000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
85
-
-
Saccharolobus solfataricus

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
30 90 30┬░C: about 90% of activity maximum, 90┬░C: about 35% of activity maximum Saccharolobus solfataricus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
70 100 no loss in activity after 1 h at 90┬░C, 20% residual activity after 1 h at 100┬░C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
NAD(P)H + 2-oxoglutarate + NH3 Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
7 8 90-100% activity Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 at least 65% residual activity after 14 h Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Saccharolobus solfataricus
NADPH
-
Saccharolobus solfataricus