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BRENDA support

1.4.1.3: glutamate dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about glutamate dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

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L-glutamate
+
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H2O
+
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NAD(P)+
=
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2-oxoglutarate
+
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NH3
+
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NAD(P)H
+
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H+

Synonyms

At5g07440, At5g18170, dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)), dual-coenzyme specific glutamate dehydrogenase, GDH, gdh-1, GDH1, GDH2, GDH3, GdhA, gdhA_1, GDHB, GDHII, GLDH, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2, hGLUD1, hGLUD2, housekeeping glutamate dehydrogenase, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, Legdh1, Membrane protein 50, MP50, NAD(P)+-dependent glutamate dehydrogenase, NAD(P)-dependent GDH, NAD(P)-dependent glutamate dehydrogenase, NAD(P)-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NAD(P)H-utilizing glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, TTC1211, TTC1212, TtGDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                EC 1.4.1.31.4.1.3 glutamate dehydrogenase [NAD(P)+]

Cloned

Cloned on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+]

for references in articles please use BRENDA:EC1.4.1.3

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
chimeric mutant enzymes expressed in Escherichia coli DE3 cells
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coexpression of wild-type and mutant enzymes with GroES and GroEl in Escherichia coli
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expressed in Escherichia coli
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expressed in Escherichia coli BL21 (DE3)
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21DE3 cells
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expressed in sf21 cells
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expressed in Sf21 cells using the baculovirus expression system
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expressed in Sf9 cells and HEK-293T cells
expression by in vitro transcription/translation in rabbit reticulocytes
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expression in Escherichia coli
expression in Escherichia coli BL21 by using the pET-15b expression vector containing a T7 promoter
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expression in Escherichia coli. The specific activity of the hexameric form of the recombinant enzyme is much lower than that of the natural enzyme. The structure of the hexameric form of the recombinant enzyme with low specific activity (Type I) is different from that of the natural enzyme with high specific activity (Type II). Upon heat treatment (80┬░C, 15 min), the Type I structure is effectively converted to Type II structure and the specific activity of the enzyme is increased by 2.6fold
expression in Saccharomyces cerevisiae
expression in Sf21 cells using a baculovirus expression system
expression in Sf9 cells
expression in Sf9 cells using the baculovirus expression system
expression of Glu279 mutants in Escherichia coli
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expression of His-tagged GDH2 wild-type and mutants in Escherichia coli
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expression of His-tagged wild-type and mutant GDHA and GDHB in Escherichia coli strain BL21-CodonPlus (DE3)-RIL, and of untagged proteins
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gene GLUD1, recombinant expression of mutant enzymes in Spodoptera frugiperda SF21 cells
gene GLUD2, expression of 12-amino acid GDH2-specific peptide PTAEFQDSISGA, corresponding to residues 436-447 of the mature human protein, in Spodoptere frugiperda Sf21 cells using the baculovirus transfection method
gene GLUD2, recombinant expression of wild-type enzyme in Spodoptera frugiperda SF21 cells
introducing gene Glud1 under the control of the neuron-specific enolase promoter into mouse brain
mutant enzymes are expressed in Escherichia coli BL21 (DE3) cells
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mutant proteins expressed in Escherichia coli as soluble proteins
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overexpression in Escherichia coli BL21
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recombinant expression of wild-type GLUD1 and GLUD2 in Spodoptera frugiperda Sf21 cells using the baculovirus expression system
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two GDH homologues, gdhA and gdhB, with 46% amino acid sequence identity, are present in a tandem manner as genes TTC1212 and TTC1211 on the genome of Thermus thermophilus, recombinant expression of the His-tagged ternary complex TtGDH-APRTh in Escherichia coli
wild-type and mutant enzyme, expression in Sf21 cells using a baculovirus expression system
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wild-type enzyme hGDH2 and mutant enzymes
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wild-type hGDH1
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