1.4.1.3: glutamate dehydrogenase [NAD(P)+]
This is an abbreviated version!
For detailed information about glutamate dehydrogenase [NAD(P)+], go to the full flat file.
Reaction
Synonyms
At5g07440, At5g18170, dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)), dual-coenzyme specific glutamate dehydrogenase, GDH, gdh-1, GDH1, GDH2, GDH3, GdhA, gdhA_1, GDHB, GDHII, GLDH, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2, hGLUD1, hGLUD2, housekeeping glutamate dehydrogenase, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, Legdh1, Membrane protein 50, MP50, NAD(P)+-dependent glutamate dehydrogenase, NAD(P)-dependent GDH, NAD(P)-dependent glutamate dehydrogenase, NAD(P)-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NAD(P)H-utilizing glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, TTC1211, TTC1212, TtGDH
ECTree
1.4.1.3 glutamate dehydrogenase [NAD(P)+]Advanced search results
results (
results (Specific Activity
Specific Activity on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+]
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SPECIFIC ACTIVITY [µmol/min/mg] 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
0.168
-
NADH dependent activity in cells grown on medium containing 1 mM NH4Cl
0.257
-
NADH dependent activity in cells grown on medium containing 1 mM NH4Cl
0.336
-
NADH dependent activity in cells grown on medium containing 1 mM NH4Cl
0.858
-
NADPH dependent activity in cells grown on medium containing 1 mM NH4Cl
1.07
-
NADH dependent activity in cells grown on medium containing 1 mM NH4Cl
1.96
-
NADPH dependent activity in cells grown on medium containing 1 mM NH4Cl
100
-
recombinant R463A mutant enzyme, maximal activity in the presence of 6 mM leucine
130
140
-
recombinant H454Y mutant enzyme, maximal activity in the presence of 0.2 mM ADP
2.22
-
NADPH dependent activity in cells grown on medium containing 1 mM NH4Cl
423
5.18
-
NADPH dependent activity in cells grown on medium containing 1 mM NH4Cl
55
-
recombinant S448P mutant enzyme, maximal activity in the presence of 6 mM leucine
69
-
recombinant S448P mutant enzyme, maximal activity in the presence of 0.2 mM ADP
additional information
130
-
recombinant wild-type enzyme, maximal activity in the presence of 0.2 mM ADP or 6 mM leucine
130
-
recombinant S448P mutant enzyme, maximal activity in the presence of 6 mM leucine
additional information
-
hGDH1 displays a high basal activity of 40% of its maximal activity. Regulation of the hGDH1 activity is achieved by interplay of GTP and ADP/L-leucine
additional information
-
hGDH2 displays a basal activity less than 10% of its maximal activity. Control of the hGDH2 activity depends on the level of its basal activity and on changing ADP/L-leucine concentrations. For hGDH2 enzyme protein concentration and basal activity show a linear pattern of dependence. Lowering the temperature of the reaction mixture from 25°C to 20°C has a stabilizing effect on hGDH2, as under these conditions, the specific basal activities of the enzyme increases. Increasing the concentration of the TrisHCl buffer from 50 mM to 125 mM or to 250 mM (pH 8.0) raises basal activity levels, but the maximal activity declines
additional information
enzyme activity in wild-type and transgenic overexpressing brain cell fractions, overview
additional information
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enzyme activity in wild-type and transgenic overexpressing brain cell fractions, overview
additional information
the specific activity of the hexameric form of the recombinant enzyme is much lower than that of the natural enzyme. The structure of the hexameric form of the recombinant enzyme with low specific activity (Type I) is different from that of the natural enzyme with high specific activity (Type II). Upon heat treatment (80°C, 15 min), the Type I structure is effectively converted to Type II structure and the specific activity of the enzyme is increased by 2.6fold
