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Literature summary for 1.4.1.3 extracted from

  • Yamamoto, I.; Abe, A.; Ishimoto, M.
    Properties of glutamate dehydrogenase purified from Bacteroides fragilis (1987), J. Biochem., 101, 1391-1397.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate
-
Bacteroides fragilis
D-glutamate 10 mM, 57% inhibition of NADP+-linked activity, 30% inhibition of NADPH-linked activity Bacteroides fragilis
glutamate
-
Bacteroides fragilis
NaCl 100 mM, 50% inhibition of NADH and NAD+ dependent reactions Bacteroides fragilis
oxaloacetate 5 mM, 20-25% inhibition of NADH- and NAD+-dependent activities Bacteroides fragilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
NADPH NADP-linked reductive amination Bacteroides fragilis
0.019
-
NADP+ NADP-linked oxidative deamination Bacteroides fragilis
0.14
-
2-oxoglutarate NADP-linked reductive amination Bacteroides fragilis
0.2
-
NADH NAD-linked reductive amination Bacteroides fragilis
1.7
-
NH3 NADP-linked reductive amination Bacteroides fragilis
2.4
-
L-glutamate NADP-linked oxidative deamination Bacteroides fragilis
3
-
NAD+ NAD-linked oxidative deamination Bacteroides fragilis
4.9
-
NH3 NAD-linked reductive amination Bacteroides fragilis
5.1
-
NH3 NADP-linked reductive amination Bacteroides fragilis
7.1
-
2-oxoglutarate NAD-linked reductive amination Bacteroides fragilis
7.3
-
L-glutamate NAD-linked oxidative deamination Bacteroides fragilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000 350000 polyacrylamide disc gel electrophoresis Bacteroides fragilis

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
strictly anaerobic bacterium
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacteroides fragilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
18.4
-
-
Bacteroides fragilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobutyrate + H2O + NADP+ 3% of activity with L-glutamate Bacteroides fragilis 2-oxobutyrate + NH3 + NADPH
-
?
2-oxoglutarate + NH3 + NADH + H+
-
Bacteroides fragilis L-glutamate + H2O + NAD+
-
r
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Bacteroides fragilis L-glutamate + H2O + NAD+
-
r
L-glutamate + H2O + NAD(P)+
-
Bacteroides fragilis 2-oxoglutarate + NH3 + NAD(P)H
-
r
L-glutamate + H2O + NAD+
-
Bacteroides fragilis 2-oxoglutarate + NH3 + NADH + H+
-
r
valine + H2O + NADP+ 3% of activity with L-glutamate Bacteroides fragilis 2-oxovalerate + NH3 + NADPH
-
?

Subunits

Subunits Comment Organism
hexamer SDS-PAGE Bacteroides fragilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
NADH + 2-oxoglutarate + NH3 Bacteroides fragilis
8
-
NADPH + 2-oxoglutarate + NH3 Bacteroides fragilis
9
-
NADH + NH3 + 2-oxoglutarate Bacteroides fragilis
9.5
-
glutamate + NADP+ Bacteroides fragilis

Cofactor

Cofactor Comment Organism Structure
NADPH amination activity is 5fold higher with NADPH than that with NADH Bacteroides fragilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
70
-
NaCl
-
Bacteroides fragilis
80
-
L-glutamate reductive amination with NADPH, not with NADH is inhibited Bacteroides fragilis