Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Rattus norvegicus | |
ADP | - |
Bos taurus | |
ADP | 1 mM, approx. 2fold activation of membrane-bound liver enzyme | Sus scrofa | |
ATP | - |
Rattus norvegicus | |
ATP | - |
Bos taurus | |
ATP | approx. 1.5fold activation of membrane-bound liver enzyme | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus | |
ATP | membrane-bound enzyme form, inhibition of microtubule-binding activity | Sus scrofa | |
GTP | - |
Bos taurus | |
GTP | - |
Rattus norvegicus | |
GTP | membrane-bound liver enzyme, complete inhibition | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | peripheral membrane protein | Sus scrofa | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
6 * 50000, membrane-bound liver enzyme, SDS-PAGE | Sus scrofa |
330000 | - |
membrane-bound liver enzyme, velocity sedimentation on sucrose density gradient | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
membrane-bound enzyme exhibits microtubule-binding activity | - |
Purification (Comment) | Organism |
---|---|
affinity-purified | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
brain | - |
Sus scrofa | - |
brain | - |
Bos taurus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.6 | - |
membrane-bound liver enzyme | Sus scrofa |
17.8 | - |
liver enzyme | Rattus norvegicus |
34 | - |
liver enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Rattus norvegicus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
L-glutamate + H2O + NAD(P)+ | - |
Sus scrofa | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
L-glutamate + H2O + NAD(P)+ | - |
Bos taurus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 50000, membrane-bound liver enzyme, SDS-PAGE | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
ATP | inhibition of microtubules to membrane-bound liver enzyme | Sus scrofa |