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Literature summary for 1.4.1.3 extracted from

  • Rajas, F.; Rousset, B.
    A membrane-bound form of glutamate dehydrogenase possesses an ATP-dependent high-affinity microtubule-binding activity (1993), Biochem. J., 295, 447-455.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ADP
-
Rattus norvegicus
ADP
-
Bos taurus
ADP 1 mM, approx. 2fold activation of membrane-bound liver enzyme Sus scrofa
ATP
-
Rattus norvegicus
ATP
-
Bos taurus
ATP approx. 1.5fold activation of membrane-bound liver enzyme Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Bos taurus
ATP membrane-bound enzyme form, inhibition of microtubule-binding activity Sus scrofa
GTP
-
Bos taurus
GTP
-
Rattus norvegicus
GTP membrane-bound liver enzyme, complete inhibition Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane peripheral membrane protein Sus scrofa 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
6 * 50000, membrane-bound liver enzyme, SDS-PAGE Sus scrofa
330000
-
membrane-bound liver enzyme, velocity sedimentation on sucrose density gradient Sus scrofa

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
membrane-bound enzyme exhibits microtubule-binding activity
-

Purification (Commentary)

Purification (Comment) Organism
affinity-purified Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
brain
-
Sus scrofa
-
brain
-
Bos taurus
-
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
4.6
-
membrane-bound liver enzyme Sus scrofa
17.8
-
liver enzyme Rattus norvegicus
34
-
liver enzyme Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
-
Rattus norvegicus 2-oxoglutarate + NH3 + NAD(P)H
-
r
L-glutamate + H2O + NAD(P)+
-
Sus scrofa 2-oxoglutarate + NH3 + NAD(P)H
-
r
L-glutamate + H2O + NAD(P)+
-
Bos taurus 2-oxoglutarate + NH3 + NAD(P)H
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 50000, membrane-bound liver enzyme, SDS-PAGE Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.76
-
ATP inhibition of microtubules to membrane-bound liver enzyme Sus scrofa