Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.3 extracted from

  • Kim, D.W.; Eum, W.S.; Jang, S.H.; Yoon, C.S.; Kim, Y.H.; Choi, S.H.; Choi, H.S.; Kim, S.Y.; Kwon, H.Y.; Kang, J.H.; Kwon, O.s.; Cho, S.w.; Park, J.; Choi, S.Y.
    Molecular gene cloning, expression, and characterization of bovine brain glutamate dehydrogenase (2003), J. Biochem. Mol. Biol., 36, 545-551.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP increases the activity up to 2.3fold Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 by using the pET-15b expression vector containing a T7 promoter Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
ATP 0.01-0.1 mM Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
NAD+ pH 8.0, 25┬░C, recombinant enzyme Bos taurus
0.11
-
NAD+ pH 8.0, 25┬░C, native enzyme Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57500
-
x * 57500, recombinant enzyme, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADH + NH3
-
Bos taurus L-glutamate + NAD+ + H2O
-
?

Subunits

Subunits Comment Organism
? x * 57500, recombinant enzyme, SDS-PAGE Bos taurus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bos taurus
NADH
-
Bos taurus