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Literature summary for 1.4.1.3 extracted from

  • Yoon, H.Y.; Cho, E.H.; Kwon, H.Y.; Choi, S.Y.; Cho, S.W.
    Importance of glutamate 279 for the coenzyme binding of human glutamate dehydrogenase (2002), J. Biol. Chem., 277, 41448-41454.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP 0.1-1.0 mM, activates wild-type enzyme and mutant enzyme E279G Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of Glu279 mutants in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E279G 9.8fold increase in Km-value for NAD+ Homo sapiens
E279L 14.1fold increase in Km-value for NAD+ Homo sapiens
E279M 14.1fold increase in Km-value for NAD+ Homo sapiens
E279R 10.7fold increase in Km-value for NAD+ Homo sapiens
E279Y 11.8fold increase in Km-value for NAD+ Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GTP inhibits wild-type enzyme and mutant enzyme E279G Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.71
-
NAD+ pH 9.5, 25┬░C, wild-type enzyme Homo sapiens
3.44
-
L-glutamate pH 9.5, 25┬░C, wild-type enzyme Homo sapiens
3.76
-
L-glutamate pH 9.5, 25┬░C, mutant enzyme E279M Homo sapiens
3.94
-
L-glutamate pH 9.5, 25┬░C, mutant enzyme E279Y Homo sapiens
3.98
-
L-glutamate pH 9.5, 25┬░C, mutant enzyme E279G Homo sapiens
4.05
-
L-glutamate pH 9.5, 25┬░C, mutant enzyme E279L Homo sapiens
4.12
-
L-glutamate pH 9.5, 25┬░C, mutant enzyme E279R Homo sapiens
6.94
-
NAD+ pH 9.5, 25┬░C, mutant enzyme E279G Homo sapiens
7.6
-
NAD+ pH 9.5, 25┬░C, mutant enzyme E279R Homo sapiens
8.35
-
NAD+ pH 9.5, 25┬░C, mutant enzyme E279Y Homo sapiens
9.98
-
NAD+ pH 9.5, 25┬░C, mutant enzyme E279M Homo sapiens
10.01
-
NAD+ pH 9.5, 25┬░C, mutant enzyme E279L Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and Glu279 mutant enzymes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + NAD+ + H2O
-
Homo sapiens 2-oxoglutarate + NADH + NH3
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
51
-
NAD+ pH 9.5, 25┬░C, mutant enzyme E279M Homo sapiens
57
-
NAD+ pH 9.5, 25┬░C, mutant enzyme E279L Homo sapiens
59
-
NAD+ pH 9.5, 25┬░C, mutant enzyme E279G Homo sapiens
61
-
NAD+ pH 9.5, 25┬░C, mutant enzyme E279R Homo sapiens
65
-
NAD+ pH 9.5, 25┬░C, wild-type enzyme Homo sapiens
68
-
NAD+ pH 9.5, 25┬░C, mutant enzyme E279Y Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens