Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.3 extracted from

  • Choi, M.M.; Kim, E.A.; Yang, S.J.; Choi, S.Y.; Cho, S.W.; Huh, J.W.
    Amino acid changes within antenna helix are responsible for different regulatory preferences of human glutamate dehydrogenase isozymes (2007), J. Biol. Chem., 282, 19510-19517.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP activation at 1 mM Homo sapiens
L-leucine
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes are expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the catalytic properties of the chimeric enzymes GDH1(GDH2390-448)GDH1 and GDH2(GDH1390-448)GDH2 are not altered compared to the wild type enzyme and show almost identical sensitivity to palmitoyl-CoA inhibitory aspects of the original wild type isozymes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GTP
-
Homo sapiens
palmitoyl-CoA concentration-dependent inhibition, GDH1 is less sensitive to palmitoyl-CoA inhibition than GDH2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
NADH isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25┬░C Homo sapiens
0.086
-
NADH isozyme GDH2, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25┬░C Homo sapiens
1.25
-
2-oxoglutarate isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25┬░C Homo sapiens
1.39
-
2-oxoglutarate isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25┬░C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2,5-ADP-Sepharose column chromatography and Resource-Q column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
-
Homo sapiens 2-oxoglutarate + NH3 + NAD(P)H + H+
-
?

Synonyms

Synonyms Comment Organism
GDH1 housekeeping isozyme Homo sapiens
GDH2 nerve-specific isozyme Homo sapiens

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
45
-
the half life of isozyme GDH1 is 310 min at 45┬░C, the half life of isozyme GDH2 is 45 min at 45┬░C Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
104
-
NADH isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25┬░C Homo sapiens
130
-
NADH isozyme GDH2, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25┬░C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
Homo sapiens