Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | activation at 1 mM | Homo sapiens | |
L-leucine | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in Escherichia coli BL21 (DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the catalytic properties of the chimeric enzymes GDH1(GDH2390-448)GDH1 and GDH2(GDH1390-448)GDH2 are not altered compared to the wild type enzyme and show almost identical sensitivity to palmitoyl-CoA inhibitory aspects of the original wild type isozymes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | - |
Homo sapiens | |
palmitoyl-CoA | concentration-dependent inhibition, GDH1 is less sensitive to palmitoyl-CoA inhibition than GDH2 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
NADH | isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C | Homo sapiens | |
0.086 | - |
NADH | isozyme GDH2, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C | Homo sapiens | |
1.25 | - |
2-oxoglutarate | isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C | Homo sapiens | |
1.39 | - |
2-oxoglutarate | isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2,5-ADP-Sepharose column chromatography and Resource-Q column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Homo sapiens | 2-oxoglutarate + NH3 + NAD(P)H + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDH1 | housekeeping isozyme | Homo sapiens |
GDH2 | nerve-specific isozyme | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the half life of isozyme GDH1 is 310 min at 45°C, the half life of isozyme GDH2 is 45 min at 45°C | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
104 | - |
NADH | isozyme GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C | Homo sapiens | |
130 | - |
NADH | isozyme GDH2, in the presence of 1 mM ADP, in 50 mM triethanolamine, pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0, at 25°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Homo sapiens |