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Literature summary for 1.4.1.3 extracted from

  • Kanavouras, K.; Mastorodemos, V.; Borompokas, N.; Spanaki, C.; Plaitakis, A.
    Properties and molecular evolution of human GLUD2 (neural and testicular tissue-specific) glutamate dehydrogenase (2007), J. Neurosci. Res., 85, 1101-1109.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP activates at 1 mM Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in sf21 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
R443S/G456A resistant to GTP inhibition Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GTP inhibits isozyme GDH1 Homo sapiens
additional information GDH2 is resistant to GTP inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
2-oxoglutarate mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP Homo sapiens
2
-
2-oxoglutarate wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP Homo sapiens
2.1
-
2-oxoglutarate wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP Homo sapiens
10.7
-
L-glutamate wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP Homo sapiens
10.8
-
L-glutamate mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP Homo sapiens
12.4
-
L-glutamate wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP Homo sapiens
13.4
-
NH3 wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP Homo sapiens
17.1
-
NH3 wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP Homo sapiens
22.2
-
NH3 mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
6 * 56000, SDS-PAGE Homo sapiens
336000
-
SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, phenyl-Sepharose column chromatography, and hydroxyapatite chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
nerve
-
Homo sapiens
-
testis
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
145.7
-
purified mutant enzyme R443S/G456A Homo sapiens
159.1
-
purified isozyme GDH2 Homo sapiens
161.9
-
purified isozyme GDH1 Homo sapiens

Storage Stability

Storage Stability Organism
5┬░C, sodium phosphate buffer pH 6.8, several months, no loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
-
Homo sapiens 2-oxoglutarate + NH3 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
homohexamer 6 * 56000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
GDH1 isozyme Homo sapiens
GDH2 isozyme Homo sapiens
GLUD2 isozyme Homo sapiens

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
47.5
-
GDH2 has a half-life of 38 min at 47.5┬░C, GDH1 has a half-life of 348 min at 47.5┬░C, high concentrations of phosphate (300 mM) have a protective effect on the thermal denaturation of both wild type hGDHs, this effect being more pronounced for GDH2 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
isozyme GDH2 Homo sapiens
7.5 8 isozyme GDH1 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.31
-
GDH1, in the absence of ADP, in 50 mM triethanolamine pH 8.0 buffer Homo sapiens GTP
18.5
-
GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine pH 8.0 buffer Homo sapiens GTP
78.5
-
GDH2, in the absence of ADP, in 50 mM triethanolamine pH 8.0 buffer in 50 mM TRA pH 8.0 buffer Homo sapiens GTP
139.4
-
mutant enzyme R443S/G456A, in the absence of ADP, in 50 mM triethanolamine pH 8.0 buffer Homo sapiens GTP
166.8
-
GDH2, in the presence of 1 mM ADP, in 50 mM triethanolamine pH 8.0 buffer Homo sapiens GTP
622.4
-
mutant enzyme R443S/G456A, in the absence of ADP, in 50 mM triethanolamine pH 8.0 buffer Homo sapiens GTP