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Literature summary for 1.4.1.3 extracted from

  • Choi, M.M.; Huh, J.W.; Yang, S.J.; Cho, E.H.; Choi, S.Y.; Cho, S.W.
    Identification of ADP-ribosylation site in human glutamate dehydrogenase isozymes (2005), FEBS Lett., 579, 4125-4130.
    View publication on PubMed
Search for more literature for 1.4.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
C119A reduction in the ADP-ribosylation Homo sapiens
C119G reduction in the ADP-ribosylation Homo sapiens
C119Y reduction in the ADP-ribosylation Homo sapiens
C274A reduction in the ADP-ribosylation Homo sapiens
C274G reduction in the ADP-ribosylation Homo sapiens
C274Y reduction in the ADP-ribosylation Homo sapiens
C59A reduction in the ADP-ribosylation Homo sapiens
C59G reduction in the ADP-ribosylation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NAD+ incubation with 0.1 mM for 60 min inhibits hGDH1 and hGDH2 by 75% and 70%, respectively, incubations for longer time periods up to 3 h, does not further increase the inhibition of hGDH isoenzymes, ADP-ribosylated hDGH isozymes are reactivated by Mg2+-dependent mitochondrial ADP-ribosylcysteine hydrolase Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
 NADH wild-type, hGDH1 Homo sapiens
0.086
-
 NADH wild-type, hGDH2 Homo sapiens
0.09
-
 NADH mutant C119A, hGDH1 Homo sapiens
0.091
-
 NADH mutant C119Y, hGDH1 Homo sapiens
0.092
-
 NADH mutant C119G, hGDH1 Homo sapiens
0.094
-
 NADH mutant C59G, hGDH1 Homo sapiens
0.097
-
 NADH mutant C59A, hGDH1 Homo sapiens
0.098
-
 NADH mutant C119G, hGDH2 Homo sapiens
0.099
-
 NADH mutant C59A, hGDH2 Homo sapiens
0.099
-
 NADH mutant C59Y, hGDH1 Homo sapiens
0.101
-
 NADH mutant C119A, hGDH2 Homo sapiens
0.102
-
 NADH mutant C59Y, hGDH2 Homo sapiens
0.106
-
 NADH mutant C59G, hGDH2 Homo sapiens
0.108
-
 NADH mutant C119Y, hGDH2 Homo sapiens
0.169
-
 NADH mutant C274A, hGDH2 Homo sapiens
0.175
-
 NADH mutant C274G, hGDH2 Homo sapiens
0.176
-
 NADH mutant C274Y, hGDH2 Homo sapiens
0.178
-
 NADH mutant C274Y, hGDH1 Homo sapiens
0.181
-
 NADH mutant C274G, hGDH1 Homo sapiens
0.189
-
 NADH mutant C274A, hGDH1 Homo sapiens
1.16
-
 2-oxoglutarate mutant C274G, hGDH1 Homo sapiens
1.25
-
 2-oxoglutarate wild-type, hGDH1 Homo sapiens
1.34
-
 2-oxoglutarate mutant C59G, hGDH1 Homo sapiens
1.36
-
 2-oxoglutarate mutant C59Y, hGDH1 Homo sapiens
1.37
-
 2-oxoglutarate mutant C274Y, hGDH1 Homo sapiens
1.39
-
 2-oxoglutarate wild-type, hGDH2 Homo sapiens
1.4
-
 2-oxoglutarate mutant C274A, hGDH1 Homo sapiens
1.4
-
 2-oxoglutarate mutant C59A, hGDH1 Homo sapiens
1.58
-
 2-oxoglutarate mutant C274G, hGDH2 Homo sapiens
1.58
-
 2-oxoglutarate mutant C59Y, hGDH2 Homo sapiens
1.59
-
 2-oxoglutarate mutant C274A, hGDH2 Homo sapiens
1.61
-
 2-oxoglutarate mutant C59A, hGDH2 Homo sapiens
1.66
-
 2-oxoglutarate mutant C274Y, hGDH2 Homo sapiens
1.67
-
 2-oxoglutarate mutant C59G, hGDH2 Homo sapiens
1.86
-
 2-oxoglutarate mutant C119Y, hGDH1 Homo sapiens
1.9
-
 2-oxoglutarate mutant C119A, hGDH1 Homo sapiens
1.94
-
 2-oxoglutarate mutant C119G, hGDH1 Homo sapiens
2.12
-
 2-oxoglutarate mutant C119A, hGDH2 Homo sapiens
2.18
-
 2-oxoglutarate mutant C119G, hGDH2 Homo sapiens
2.26
-
 2-oxoglutarate mutant C119Y, hGDH2 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADH + NH3
-
 Homo sapiens L-glutamate + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
GDH
-
 Homo sapiens
glutamate dehydrogenase
-
 Homo sapiens
hGDH1
-
 Homo sapiens
hGDH2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
77
-
 NADH mutant C119G, hGDH1 Homo sapiens
79
-
 NADH mutant C119Y, hGDH1 Homo sapiens
81
-
 NADH mutant C119A, hGDH1 Homo sapiens
85
-
 NADH mutant C59Y, hGDH1 Homo sapiens
88
-
 NADH mutant C274Y, hGDH1 Homo sapiens
90
-
 NADH mutant C274A, hGDH1 Homo sapiens
90
-
 NADH mutant C59A, hGDH1 Homo sapiens
91
-
 NADH mutant C274G, hGDH1 Homo sapiens
91
-
 NADH mutant C59G, hGDH1 Homo sapiens
100
-
 NADH mutant C274Y, hGDH2 Homo sapiens
101
-
 NADH mutant C119G, hGDH2 Homo sapiens
103
-
 NADH mutant C119Y, hGDH2 Homo sapiens
104
-
 NADH wild-type, hGDH1 Homo sapiens
105
-
 NADH mutant C119A, hGDH2 Homo sapiens
108
-
 NADH mutant C59Y, hGDH2 Homo sapiens
109
-
 NADH mutant C274G, hGDH2 Homo sapiens
110
-
 NADH mutant C59G, hGDH2 Homo sapiens
114
-
 NADH mutant C274A, hGDH2 Homo sapiens
117
-
 NADH mutant C59A, hGDH2 Homo sapiens
130
-
 NADH wild-type, hGDH2 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Homo sapiens