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Literature summary for 1.4.1.3 extracted from
- Glutamate dehydrogenase isoforms with N-terminal (His)6- or FLAG-tag retain their kinetic properties and cellular localization (2014), Neurochem. Res., 39, 487-499 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | the addition of FLAG tag to the C-terminus of GDH leaves the recombinant protein fivefold less sensitive to ADP activation | Mus musculus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sf9 cells and HEK-293T cells | Homo sapiens |
| expressed in Sf9 cells and HEK-293T cells | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | neither FLAG nor (His)6 tags disturb the mitochondrial localization of GDH. The addition of the small tags to the N-terminus of the mature mitochondrial enzyme does not change the ADP activation or GTP inhibition pattern of the proteins | Homo sapiens |
| additional information | neither FLAG nor (His)6 tags disturb the mitochondrial localization of GDH. The addition of the small tags to the N-terminus of the mature mitochondrial enzyme does not change the ADP activation or GTP inhibition pattern of the proteins. The addition of FLAG tag to the C-terminus of the mouse GDH left the recombinant protein fivefold less sensitive to ADP activation | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GTP | - |
Homo sapiens | |
| GTP | - |
Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
| mitochondrion | - |
Mus musculus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P00367 | - |
- |
| Homo sapiens | P49448 | - |
- |
| Mus musculus | P26443 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Homo sapiens |
| recombinant protein | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDH1 | - |
Homo sapiens |
| GDH2 | - |
Homo sapiens |
| GDH2 | - |
Mus musculus |
| GLUD1 | - |
Homo sapiens |
| GLUD2 | - |
Homo sapiens |
| GLUD2 | - |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | cofactor specificity not mentioned | Homo sapiens | |
| additional information | cofactor specificity not mentioned | Mus musculus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0106 | - |
C-terminally FLAG-tagged recombinant protein, pH 8.0, 23°C | Mus musculus | GTP | |
| 0.0196 | - |
His-tagged recombinant protein, pH 8.0, 23°C | Homo sapiens | GTP | |
| 0.0212 | - |
wild-type, pH 8.0, 23°C | Homo sapiens | GTP | |
| 0.0245 | - |
wild-type, pH 8.0, 23°C | Mus musculus | GTP | |
| 0.0271 | - |
N-terminally FLAG-tagged recombinant protein, pH 8.0, 23°C | Mus musculus | GTP | |
| 0.242 | - |
wild-type, pH 8.0, 23°C | Homo sapiens | GTP | |
| 0.25 | - |
His-tagged recombinant protein, pH 8.0, 23°C | Homo sapiens | GTP | |
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