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Literature summary for 1.4.1.3 extracted from

  • Pajecka, K.; Nielsen, C.W.; Hauge, A.; Zaganas, I.; Bak, L.K.; Schousboe, A.; Plaitakis, A.; Waagepetersen, H.S.
    Glutamate dehydrogenase isoforms with N-terminal (His)6- or FLAG-tag retain their kinetic properties and cellular localization (2014), Neurochem. Res., 39, 487-499 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP the addition of FLAG tag to the C-terminus of GDH leaves the recombinant protein fivefold less sensitive to ADP activation Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 cells and HEK-293T cells Homo sapiens
expressed in Sf9 cells and HEK-293T cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information neither FLAG nor (His)6 tags disturb the mitochondrial localization of GDH. The addition of the small tags to the N-terminus of the mature mitochondrial enzyme does not change the ADP activation or GTP inhibition pattern of the proteins Homo sapiens
additional information neither FLAG nor (His)6 tags disturb the mitochondrial localization of GDH. The addition of the small tags to the N-terminus of the mature mitochondrial enzyme does not change the ADP activation or GTP inhibition pattern of the proteins. The addition of FLAG tag to the C-terminus of the mouse GDH left the recombinant protein fivefold less sensitive to ADP activation Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
GTP
-
Homo sapiens
GTP
-
Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-
mitochondrion
-
Mus musculus 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P00367
-
-
Homo sapiens P49448
-
-
Mus musculus P26443
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Homo sapiens
recombinant protein Mus musculus

Synonyms

Synonyms Comment Organism
GDH1
-
Homo sapiens
GDH2
-
Homo sapiens
GDH2
-
Mus musculus
GLUD1
-
Homo sapiens
GLUD2
-
Homo sapiens
GLUD2
-
Mus musculus

Cofactor

Cofactor Comment Organism Structure
additional information cofactor specificity not mentioned Homo sapiens
additional information cofactor specificity not mentioned Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0106
-
C-terminally FLAG-tagged recombinant protein, pH 8.0, 23┬░C Mus musculus GTP
0.0196
-
His-tagged recombinant protein, pH 8.0, 23┬░C Homo sapiens GTP
0.0212
-
wild-type, pH 8.0, 23┬░C Homo sapiens GTP
0.0245
-
wild-type, pH 8.0, 23┬░C Mus musculus GTP
0.0271
-
N-terminally FLAG-tagged recombinant protein, pH 8.0, 23┬░C Mus musculus GTP
0.242
-
wild-type, pH 8.0, 23┬░C Homo sapiens GTP
0.25
-
His-tagged recombinant protein, pH 8.0, 23┬░C Homo sapiens GTP