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Literature summary for 1.4.1.3 extracted from

  • Treberg, J.R.; Brosnan, M.E.; Brosnan, J.T.
    The simultaneous determination of NAD(H) and NADP(H) utilization by glutamate dehydrogenase (2010), Mol. Cell. Biochem., 344, 253-259.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP allosteric activator, addition of ADP or leucine to the single cofactor assay results in a marked activation of NADPH oxidation, about 1100% activation by ADP. Relative activation by ADP of GDH-catalyzed NAD+reduction is 36%, compared with 198% for NADP+ reduction Bos taurus
L-leucine allosteric activator, addition of ADP or leucine to the single cofactor assay results in a marked activation of NADPH oxidation, about 725% activation by L-leucine, respectively. Activation of NAD+ and NADP+ reduction by 40% and 135%, respectively Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
GTP
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Bos taurus
-
2-oxoglutarate + NH3 + NADH + H+
-
r
L-glutamate + H2O + NADP+ Bos taurus
-
2-oxoglutarate + NH3 + NADPH + H+
-
r
additional information Bos taurus the similarity in relative activation when both cofactors are present, combined with consistently greater GDH product formation from equimolar NADH than with NADPH, does not support the idea that there is a preferential utilization of NADPH by bovine GDH ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
Bos taurus 2-oxoglutarate + NH3 + NADH + H+
-
r
L-glutamate + H2O + NADP+
-
Bos taurus 2-oxoglutarate + NH3 + NADPH + H+
-
r
additional information the similarity in relative activation when both cofactors are present, combined with consistently greater GDH product formation from equimolar NADH than with NADPH, does not support the idea that there is a preferential utilization of NADPH by bovine GDH Bos taurus ?
-
?

Synonyms

Synonyms Comment Organism
GDH
-
Bos taurus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
additional information the similarity in relative activation when both cofactors are present, combined with consistently greater GDH product formation from equimolar NADH than with NADPH, does not support the idea that there is a preferential utilization of NADPH by bovine GDH. In the reductive amination direction, the rates of product formation are always greater for NADH oxidation than NADPH oxidation Bos taurus
NAD+
-
Bos taurus
NADH
-
Bos taurus
NADP+
-
Bos taurus
NADPH
-
Bos taurus

General Information

General Information Comment Organism
physiological function allosteric activation and inhibition is important for enzyme regulation, overview Bos taurus