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Literature summary for 1.4.1.3 extracted from

  • Moyano, E.; Cardenas, J.; Munoz-Blanco, J.
    Purification and properties of three NAD(P)+ isozymes of L-glutamate dehydrogenase of Chlamydomonas reinhardtii (1992), Biochim. Biophys. Acta, 1119, 63-68.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Aminooxyacetate 5 mM, weak inhibition of isozymes 1-3 Chlamydomonas reinhardtii
EDTA 5 mM, 80-90% inhibition of isozymes 1-3 Chlamydomonas reinhardtii
KCN 50 mM, strong inhibition of isozymes 1-3 Chlamydomonas reinhardtii
Methylacetimidate 100 mM, moderate inhibition of isozymes 1-3 Chlamydomonas reinhardtii
Ni2+ 1 mM, moderate inhibition of isozymes 1-3 Chlamydomonas reinhardtii
o-phenanthroline 5 mM, strong inhibition of isozymes 1-3 Chlamydomonas reinhardtii
p-hydroxymercuribenzoate 5 mM, moderate inhibition of isozymes 1-3 Chlamydomonas reinhardtii
pyridoxal 5'-phosphate 5 mM, 100% inhibition Chlamydomonas reinhardtii
Zn2+ 1 mM, strong inhibition of isozymes 1-3 Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
NADH isozyme 1, amination activity, cofactor NADH Chlamydomonas reinhardtii
0.09
-
NADH isozyme 3, amination activity, cofactor NADH Chlamydomonas reinhardtii
0.14
-
NADH isozyme 2, amination activity, cofactor NADH Chlamydomonas reinhardtii
0.2
-
NAD+ isozyme 1, deamination activity Chlamydomonas reinhardtii
0.26
-
NAD+ isozyme 2, deamination activity Chlamydomonas reinhardtii
0.27
-
NADPH isozyme 1, amination activity, cofactor NADPH Chlamydomonas reinhardtii
0.32
-
NAD+ isozyme 3, deamination activity Chlamydomonas reinhardtii
0.36
-
2-oxoglutarate isozyme 1, amination activity, cofactor NADH Chlamydomonas reinhardtii
0.46
-
NADPH isozyme 3, amination activity, cofactor NADPH Chlamydomonas reinhardtii
0.59
-
2-oxoglutarate isozyme 3, amination activity, cofactor NADH Chlamydomonas reinhardtii
0.64
-
L-glutamate isozyme 1, deamination activity Chlamydomonas reinhardtii
0.78
-
NADPH isozyme 2, amination activity, cofactor NADPH Chlamydomonas reinhardtii
1.23
-
2-oxoglutarate isozyme 2, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.54
-
2-oxoglutarate isozyme 1, amination activity, cofactor NADPH Chlamydomonas reinhardtii
1.73
-
2-oxoglutarate isozyme 3, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.85
-
2-oxoglutarate isozyme 2, amination activity, cofactor NADPH Chlamydomonas reinhardtii
3
-
L-glutamate isozyme 3, deamination activity Chlamydomonas reinhardtii
3.52
-
L-glutamate isozyme 2, deamination activity Chlamydomonas reinhardtii
13
-
NH4+ isozyme 2, amination activity, cofactor NADH Chlamydomonas reinhardtii
30
-
NH4+ isozyme 1, amination activity, cofactor NADH Chlamydomonas reinhardtii
36
-
NH4+ isozyme 1, amination activity, cofactor NADPH Chlamydomonas reinhardtii
41
-
NH4+ isozyme 3, amination activity, cofactor NADPH Chlamydomonas reinhardtii
44
-
NH4+ isozyme 3, amination activity, cofactor NADH Chlamydomonas reinhardtii
53
-
NH4+ isozyme 2, amination activity, cofactor NADPH Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 1.5fold activation of isozymes 1 and 3, 2.5fold activation of isozyme 2 Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
4 * 44000 + 2 * 46000, isoenzyme GDH2, SDS-PAGE Chlamydomonas reinhardtii
46000
-
4 * 44000 + 2 * 46000, isoenzyme GDH2, SDS-PAGE Chlamydomonas reinhardtii
266000 269000 gel filtration Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzymes, glutamate dehydrogenases 1, 2 and 3 Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
58
-
isozyme 3 Chlamydomonas reinhardtii
67
-
isozyme 2 Chlamydomonas reinhardtii
230
-
isozyme 1 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+
-
Chlamydomonas reinhardtii L-glutamate + H2O + NAD+
-
r
L-glutamate + H2O + NAD(P)+
-
Chlamydomonas reinhardtii 2-oxoglutarate + NH3 + NAD(P)H
-
r

Subunits

Subunits Comment Organism
hexamer 4 * 44000 + 2 * 46000, isoenzyme GDH2, SDS-PAGE Chlamydomonas reinhardtii
hexamer isoenzyme GDH1, SDS-PAGE Chlamydomonas reinhardtii

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
60 62
-
Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
NADPH + 2-oxoglutarate + NH3 Chlamydomonas reinhardtii
8.5
-
NADH + 2-oxoglutarate + NH3 Chlamydomonas reinhardtii
9
-
NAD+ + glutamate Chlamydomonas reinhardtii