Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aminooxyacetate | 5 mM, weak inhibition of isozymes 1-3 | Chlamydomonas reinhardtii | |
EDTA | 5 mM, 80-90% inhibition of isozymes 1-3 | Chlamydomonas reinhardtii | |
KCN | 50 mM, strong inhibition of isozymes 1-3 | Chlamydomonas reinhardtii | |
Methylacetimidate | 100 mM, moderate inhibition of isozymes 1-3 | Chlamydomonas reinhardtii | |
Ni2+ | 1 mM, moderate inhibition of isozymes 1-3 | Chlamydomonas reinhardtii | |
o-phenanthroline | 5 mM, strong inhibition of isozymes 1-3 | Chlamydomonas reinhardtii | |
p-hydroxymercuribenzoate | 5 mM, moderate inhibition of isozymes 1-3 | Chlamydomonas reinhardtii | |
pyridoxal 5'-phosphate | 5 mM, 100% inhibition | Chlamydomonas reinhardtii | |
Zn2+ | 1 mM, strong inhibition of isozymes 1-3 | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NADH | isozyme 1, amination activity, cofactor NADH | Chlamydomonas reinhardtii | |
0.09 | - |
NADH | isozyme 3, amination activity, cofactor NADH | Chlamydomonas reinhardtii | |
0.14 | - |
NADH | isozyme 2, amination activity, cofactor NADH | Chlamydomonas reinhardtii | |
0.2 | - |
NAD+ | isozyme 1, deamination activity | Chlamydomonas reinhardtii | |
0.26 | - |
NAD+ | isozyme 2, deamination activity | Chlamydomonas reinhardtii | |
0.27 | - |
NADPH | isozyme 1, amination activity, cofactor NADPH | Chlamydomonas reinhardtii | |
0.32 | - |
NAD+ | isozyme 3, deamination activity | Chlamydomonas reinhardtii | |
0.36 | - |
2-oxoglutarate | isozyme 1, amination activity, cofactor NADH | Chlamydomonas reinhardtii | |
0.46 | - |
NADPH | isozyme 3, amination activity, cofactor NADPH | Chlamydomonas reinhardtii | |
0.59 | - |
2-oxoglutarate | isozyme 3, amination activity, cofactor NADH | Chlamydomonas reinhardtii | |
0.64 | - |
L-glutamate | isozyme 1, deamination activity | Chlamydomonas reinhardtii | |
0.78 | - |
NADPH | isozyme 2, amination activity, cofactor NADPH | Chlamydomonas reinhardtii | |
1.23 | - |
2-oxoglutarate | isozyme 2, amination activity, cofactor NADH | Chlamydomonas reinhardtii | |
1.54 | - |
2-oxoglutarate | isozyme 1, amination activity, cofactor NADPH | Chlamydomonas reinhardtii | |
1.73 | - |
2-oxoglutarate | isozyme 3, amination activity, cofactor NADH | Chlamydomonas reinhardtii | |
1.85 | - |
2-oxoglutarate | isozyme 2, amination activity, cofactor NADPH | Chlamydomonas reinhardtii | |
3 | - |
L-glutamate | isozyme 3, deamination activity | Chlamydomonas reinhardtii | |
3.52 | - |
L-glutamate | isozyme 2, deamination activity | Chlamydomonas reinhardtii | |
13 | - |
NH4+ | isozyme 2, amination activity, cofactor NADH | Chlamydomonas reinhardtii | |
30 | - |
NH4+ | isozyme 1, amination activity, cofactor NADH | Chlamydomonas reinhardtii | |
36 | - |
NH4+ | isozyme 1, amination activity, cofactor NADPH | Chlamydomonas reinhardtii | |
41 | - |
NH4+ | isozyme 3, amination activity, cofactor NADPH | Chlamydomonas reinhardtii | |
44 | - |
NH4+ | isozyme 3, amination activity, cofactor NADH | Chlamydomonas reinhardtii | |
53 | - |
NH4+ | isozyme 2, amination activity, cofactor NADPH | Chlamydomonas reinhardtii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 1.5fold activation of isozymes 1 and 3, 2.5fold activation of isozyme 2 | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
4 * 44000 + 2 * 46000, isoenzyme GDH2, SDS-PAGE | Chlamydomonas reinhardtii |
46000 | - |
4 * 44000 + 2 * 46000, isoenzyme GDH2, SDS-PAGE | Chlamydomonas reinhardtii |
266000 | 269000 | gel filtration | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoenzymes, glutamate dehydrogenases 1, 2 and 3 | Chlamydomonas reinhardtii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
58 | - |
isozyme 3 | Chlamydomonas reinhardtii |
67 | - |
isozyme 2 | Chlamydomonas reinhardtii |
230 | - |
isozyme 1 | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH + H+ | - |
Chlamydomonas reinhardtii | L-glutamate + H2O + NAD+ | - |
r | |
L-glutamate + H2O + NAD(P)+ | - |
Chlamydomonas reinhardtii | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 4 * 44000 + 2 * 46000, isoenzyme GDH2, SDS-PAGE | Chlamydomonas reinhardtii |
hexamer | isoenzyme GDH1, SDS-PAGE | Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 62 | - |
Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
NADPH + 2-oxoglutarate + NH3 | Chlamydomonas reinhardtii |
8.5 | - |
NADH + 2-oxoglutarate + NH3 | Chlamydomonas reinhardtii |
9 | - |
NAD+ + glutamate | Chlamydomonas reinhardtii |