Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

5.6.2.1: DNA topoisomerase

This is an abbreviated version!
For detailed information about DNA topoisomerase, go to the full flat file.

Word Map on EC 5.6.2.1

Reaction

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining =

Synonyms

ATP-independent type I topoisomerase, AtTop1, bcTopo I, bcTopo IIIbeta, bi-subunit topoisomerase I, Bi-subunit topoisomerase IB, Ca-Top1, CrTop1, Dam DNA topoisomerase III, Deoxiribonucleate topoisomerase, Deoxiribonucleic topoisomerase, DNA Top IB, DNA topo I, DNA topoisomerase, DNA topoisomerase 1, DNA topoisomerase I, DNA topoisomerase I alpha, DNA topoisomerase I-B, DNA topoisomerase IB, DNA topoisomerase III, DNA topoisomerase IIIalpha, DNA topoisomerase type I, EC 5.99.1.2, EcTOP, ETOP, HssTopoI, HsTop1, hTop1p, hTopI, hTopo, htopo I, hTopo IIIalpha, hTopoI, human DNA topoisomerase I, human Top1, human topo I, human topoisomerase I, Isomerase, deoxiribonucleate topo-, Late protein H6, LdTop1, LdTOP1L, LdTOP1LS, LdTOP1S, LdTopIIIbeta, MimiTopIB, MsTopA, MtbTopA, mTopoI, MtTOP1, MttopoI, Nicking-closing enzyme, OjTop1, OlTop1, omega-Protein, OpTop1, PfTopI, PfTopoI, Poxviridae topoisomerase IB, Relaxing enzyme, Reverse gyrase, SiRe_1176, SsTop3, Swivelase, top I, Top1, TOP1alpha, TOP1mt, Top1p, TopA, TopI, TopIB, topo 1, Topo I, topo IB, topo V, topoI, Topoisomerase, topoisomerase 1, topoisomerase 1alpha, Topoisomerase I, topoisomerase I homolog, topoisomerase IB, topoisomerase III, topoisomerase IIIalpha, topoisomerase IIIbeta, topoisomerase IV, topoisomerase V, topoisomerase-I, topoIV, TopR1, TpI, Type I DNA topoisomerase, type I topoisomerase, type IA DNA topoisomerase, type IA topoisomerase, type IB topoisomerase, type IB topoisomerase V, Untwisting enzyme, Vaccinia DNA topoisomerase I, variola topoisomerase IB, vTopo, yeast DNA topoisomerase I, yeastTop1, YpTOP, YrdD, YTOP

ECTree

     5 Isomerases
         5.6 Isomerases altering macromolecular conformation
             5.6.2 Enzymes altering nucleic acid conformation
                5.6.2.1 DNA topoisomerase

Purification

Purification on EC 5.6.2.1 - DNA topoisomerase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
anti-FLAG M2 affinity gel column chromatography
by combination of phosphocellulose, hydroxylapitite and ssDNA agarose column chromatography as described elsewhere
-
chitin affinity chromatography followed by chromatography on hydroxyapatite
enzyme overexpressed in yeast
-
following the protocols for the purification of ecTopo III
from a soluble bacterial lysate by Ni2+-agarose chromatography
-
His6-tagged recombinant enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, cleavage and elimination of the His-tag
-
HiTrap Q HP column and SDS-PAGE
Human Topo I purified from extracts of baculovirus-infected Sf9 or Hi-5 cells
-
mutant enzymes
N-terminally epitope-tagged with sequence DYKDDDY, i.e. FLAG-tag, recombinant enzyme from Saccharomyces cerevisiae strain EKY3 by immunoaffinity chromatography
-
native enzyme partially from Hut 78 cells by nuclear extract preparation
-
Ni Sepharose 6 column chromatography and single-stranded DNA cellulose column chromatography
-
Ni2+-NTA agarose column followed by phosphocellulose column. Concentrations of purified proteins quantified by Bradford reaction
-
partial
phosphocellulose chromatography
-
phosphocellulose column chromatography and FLAG affinity column chromatography
-
Protein constructs. Structure of recombinant Leishmania donovani topoisomerase I proteins
-
Proteins are purified through Ni-NTA (Ni2+-nitrilotriacetate)–agarose or GST (glutathione transferase)–Sepharose 4B column followed by phosphocellulose column (P11 cellulose)
-
recombinant enzyme by polymin P and ammonium sulfate fractionations, cation- and anion-exchange chromatography, purified DNA topoisomerase I is free of DNase contamination, method development, overview
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration
-
recombinant His-tagged full-length enzyme and enzyme fragments from Saccharomyces cerevisiae by nickel affinity chromatography and cation exchange chromatography
-
recombinant His-tagged TopoI from Escherichia coli strain DH5alpha by ammonium sulfate fractionation and nickel affinity chromatography
recombinant His-tagged topoisomerase I mutants from Escherichia coli strain JD5 and purification by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21AI by nickel affinity chromatography
-
recombinant nontagged enzyme from Escherichia coli strain BL21 by ammonium sulfate fractionation, dialysis, and heparin affinity chromatography, recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, dialysis, and nickel affinity chromatography, native enzyme from Mycobacterium tuberculosis strain H37Ra
recombinant tagged enzyme from Escherichia coli strain BL21(DE3) by heparin affinity chromatography
-
recombinant Yersinia pestis topoisomerase I proteins with the C-terminus hexahistidine tag are purified using the ProPur IMAC Midi spin column
-
single-stranded DNA cellulose column
strategy for purifying eukaryotic type I topoisomerases
-
three truncated Topo V polypeptides comprising the NH2-terminal 44000 Da and 16000 Da domains, the 44000 Da, 16000 Da and 18000 Da domains and the C-terminal 34000 Da domain
-
topoisomerase I and several of its partial fragments
-
use of immobilized triazine dyes in purification
-
wild-type enzyme and mutant enzymes Y288F and C559A/C561A