Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.1 extracted from

  • Kieber, J.J.; Lopez, M.F.; Tissier, A.F.; Signer, E.
    Purification and properties of DNA topoisomerase I from broccoli (1992), Plant Mol. Biol., 18, 865-871.
    View publication on PubMed
Search for more literature for 5.6.2.1

Inhibitors

Inhibitors Comment Organism Structure
camptothecins
-
 Brassica oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no requirement for Mg2+ Brassica oleracea
Na+ weak stimulation by NaCl, optimal concentration: 25 mM Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration, the purified protein represents a proteolytic fragment of a larger protein Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
 var. italica
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brassica oleracea

Storage Stability

Storage Stability Organism
-20°C, stable for at least several months Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
supercoiled DNA relaxation Brassica oleracea relaxed closed circular DNA
-
 ?
supercoiled DNA positive and negative supercoiled DNA Brassica oleracea relaxed closed circular DNA
-
 ?
supercoiled DNA removes both positive and negative supercoils in steps of one Brassica oleracea relaxed closed circular DNA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Brassica oleracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Brassica oleracea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
16 42 active in this range Brassica oleracea