Protein Variants | Comment | Organism |
---|---|---|
N726D | self-poisoning phenotype, reduced rate of DNA religation relative to wild-type enzyme, affinity for DNA is reduced about 2.5fold relative to that observed for wild-type Top1p at 50 or 75 mM KCl | Saccharomyces cerevisiae |
N726F | specific activity is about 5fold lower than that of wild-type enzyme, self-poisoning phenotype, mutation enhances the kinetics of DNA cleavage | Saccharomyces cerevisiae |
N726H | self-poisoning phenotype, specific activity is about 5fold lower than that of wild-type enzyme, binding of DNA increases from 2.5fold at 50 mM KCl to 4fold at 100 mM KCl, 10fold increase in DNA cleavage activity | Saccharomyces cerevisiae |
N726K | camptothecin resistance of cells expressing the mutant enzyme | Saccharomyces cerevisiae |
N726Q | camptothecin resistance of cells expressing the mutant enzyme | Saccharomyces cerevisiae |
N726Y | mutation enhances the kinetics of DNA cleavage | Saccharomyces cerevisiae |
T722A | self-poisoning phenotype, reduced rate of DNA religation relative to wild-type enzyme, specific activity of the mutant enzyme is slightly decreased, however protein binding of DNA and rates of enzyme-catalyzed | Saccharomyces cerevisiae |
T722A/N726H | specific activity is about 5fold lower than that of wild-type enzyme, N726H mutation potentiates the cytotoxic activity of T722A, increase in DNA cleavage activity | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Top1p | - |
Saccharomyces cerevisiae |