Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-RIL | Acanthamoeba polyphaga Mimivirus |
Crystallization (Comment) | Organism |
---|---|
- |
Acanthamoeba polyphaga Mimivirus |
Protein Variants | Comment | Organism |
---|---|---|
H285A | display a low level of supercoil relaxation. Slow the rate of cleavage by a factor of 60 compared to the wild-type | Acanthamoeba polyphaga Mimivirus |
additional information | conclude that the active sites of MimiTopIB and vaccinia TopIB are composed of similar functional groups | Acanthamoeba polyphaga Mimivirus |
R135A | form no detectable protein-DNA adduct during a 24 h incubation with the suicide substrate. The failure of this protein to relax supercoiled DNA is probably attributable to its inability to perform transesterification chemistry | Acanthamoeba polyphaga Mimivirus |
R241A | form no detectable protein-DNA adduct during a 24 h incubation with the suicide substrate. The failure of this protein to relax supercoiled DNA is probably attributable to its inability to perform transesterification chemistry | Acanthamoeba polyphaga Mimivirus |
Y294A | form no detectable protein-DNA adduct during a 24 h incubation with the suicide substrate. The failure of this protein to relax supercoiled DNA is probably attributable to its inability to perform transesterification chemistry | Acanthamoeba polyphaga Mimivirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
SDS-PAGE | Acanthamoeba polyphaga Mimivirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthamoeba polyphaga Mimivirus | - |
a parasite of Acanthamoeba polyphaga | - |
Purification (Comment) | Organism |
---|---|
from a soluble bacterial lysate by Ni2+-agarose chromatography | Acanthamoeba polyphaga Mimivirus |
Subunits | Comment | Organism |
---|---|---|
monomer | The sedimentation results are consistent with MimiTopIB being a monomer in solution, although the prospect that MimiTopIB is an asymmetrically shaped homodimer cannot excluded | Acanthamoeba polyphaga Mimivirus |
Synonyms | Comment | Organism |
---|---|---|
DNA topoisomerase IB | - |
Acanthamoeba polyphaga Mimivirus |
MimiTopIB | - |
Acanthamoeba polyphaga Mimivirus |
TopIB | - |
Acanthamoeba polyphaga Mimivirus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Acanthamoeba polyphaga Mimivirus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Acanthamoeba polyphaga Mimivirus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | relaxes DNA over a broad pH range | Acanthamoeba polyphaga Mimivirus |