Cloned (Comment) | Organism |
---|---|
BL21(alphatopB) cells | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | An experiment was performed to assess the ability of bcTopo IIIbeta to substitute for ecTopo III in vivo. bcTopo IIIbeta can not substitute for the loss of ecTopo III in Escherichia coli | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Effect of Mg2+ on bcTopo IIIbeta and ecTopo III-mediated DNA relaxation assays, high concentrations of Mg2+ (>2 mM) inhibit the relaxation | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
- |
Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | Q81IH1 | ATCC14579 | - |
Bacillus cereus ATCC 14579 | Q81IH1 | ATCC14579 | - |
Purification (Comment) | Organism |
---|---|
following the protocols for the purification of ecTopo III | Bacillus cereus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining | bcTopo IIIbeta does not act on partially relaxed DNA. In contrast to ecTopo III Escherichia coli topoisomerase bcTopo IIIbeta can not further relax this substrate. bcTopo IIIbeta and ecTopo III exhibit comparable single stranded DNA binding activities and cleavage site specificities. bcTopo IIIbeta does not possess decatenation activity. Unlike ecTopo III, bcTopo III beta is not capable of decatenating replication intermediates | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
bcTopo IIIbeta | - |
Bacillus cereus |
topoisomerase IIIbeta | subfamily of prokaryotic type IA topoisomerases that are different from the prototypical ecTopo I and ecTopo III-like enzymes | Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
assay at | Bacillus cereus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 65 | Effect of temperature on bcTopo IIIbeta mediated DNA relaxation assay. Comparison with ecTopo III Escherichia coli topoisomerase characteristics | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.8 | - |
optimal pH for the bcTopo IIIbeta-mediated superhelical DNA relaxation is 9.8 or higher | Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.4 | bcTopo IIIbeta relaxes supercoiled DNA in a pH dependent manner. Distributive patterns of partially relaxed topoisomers appear with an increase of pH. Comparison with ecTopo III Escherichia coli topoisomerase characteristics | Bacillus cereus |