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Literature summary for 5.6.2.1 extracted from

  • Li, Z.; Hiasa, H.; DiGate, R.
    Characterization of a unique type IA topoisomerase in Bacillus cereus (2006), Mol. Microbiol., 60, 140-151.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
BL21(alphatopB) cells Bacillus cereus

Protein Variants

Protein Variants Comment Organism
additional information An experiment was performed to assess the ability of bcTopo IIIbeta to substitute for ecTopo III in vivo. bcTopo IIIbeta can not substitute for the loss of ecTopo III in Escherichia coli Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ Effect of Mg2+ on bcTopo IIIbeta and ecTopo III-mediated DNA relaxation assays, high concentrations of Mg2+ (>2 mM) inhibit the relaxation Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
-
Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus Q81IH1 ATCC14579
-
Bacillus cereus ATCC 14579 Q81IH1 ATCC14579
-

Purification (Commentary)

Purification (Comment) Organism
following the protocols for the purification of ecTopo III Bacillus cereus

Reaction

Reaction Comment Organism Reaction ID
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining bcTopo IIIbeta does not act on partially relaxed DNA. In contrast to ecTopo III Escherichia coli topoisomerase bcTopo IIIbeta can not further relax this substrate. bcTopo IIIbeta and ecTopo III exhibit comparable single stranded DNA binding activities and cleavage site specificities. bcTopo IIIbeta does not possess decatenation activity. Unlike ecTopo III, bcTopo III beta is not capable of decatenating replication intermediates Bacillus cereus

Synonyms

Synonyms Comment Organism
bcTopo IIIbeta
-
Bacillus cereus
topoisomerase IIIbeta subfamily of prokaryotic type IA topoisomerases that are different from the prototypical ecTopo I and ecTopo III-like enzymes Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
assay at Bacillus cereus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 65 Effect of temperature on bcTopo IIIbeta mediated DNA relaxation assay. Comparison with ecTopo III Escherichia coli topoisomerase characteristics Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.8
-
optimal pH for the bcTopo IIIbeta-mediated superhelical DNA relaxation is 9.8 or higher Bacillus cereus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10.4 bcTopo IIIbeta relaxes supercoiled DNA in a pH dependent manner. Distributive patterns of partially relaxed topoisomers appear with an increase of pH. Comparison with ecTopo III Escherichia coli topoisomerase characteristics Bacillus cereus