Literature summary for 5.6.2.1 extracted from

Roy, A.; Das, B.B.; Ganguly, A.; Bosedasgupta, S.; Khalkho, N.V.; Pal, C.; Dey, S.; Giri, V.S.; Jaisankar, P.; Dey, S.; Majumder, H.K.
An insight into the mechanism of inhibition of unusual bi-subunit topoisomerase I from Leishmania donovani by 3,3-diindolylmethane, a novel DNA topoisomerase I poison with a strong binding affinity to enzyme (2008), Biochem. J., 409, 611-622.

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Application

Application	Comment	Organism
drug development	3,3-diindolylmethane is a novel Leishmania donovani topoisomerase I poison which acts as a noncompetitive inhibitor. So this intriguing dietary component might be exploited for therapeutic development against leishmaniasis	Leishmania donovani

Cloned(Commentary)

Cloned (Comment)	Organism
Escherichia coli BL21 (DE3)	Leishmania donovani

Inhibitors

Inhibitors	Comment	Organism	Structure
3,3'-diindolylmethane	(DIM) novel DNA topoisomerase I poison, which is a class I noncompetitive inhibitor, strongly inhibits Leishmania donovani topoisomerase I and stabilizes topoisomerase I-DNA cleavable complex	Leishmania donovani
camptothecin	most widely studied inhibitor of type IB topoisomerase	Leishmania donovani
dihydrobetulinic acid	catalytic inhibitor of topoisomerase I, inhibits the suicidal cleavage reaction	Leishmania donovani

Organism

Organism	UniProt	Comment	Textmining
Leishmania donovani	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Leishmania donovani

Subunits

Subunits	Comment	Organism
heterodimer	DNA topoisomerase I of kinetoplastid protozoan parasite Leishmania donovani is an unusual bi-subunit enzyme. To find out the differential affinity of 3,3-diindolylmethane towards the large subunit (LdTOP1L) and small subunit (LdTOP1S) of Leishmania donovani topoisomerase I, performed the binding of individual subunits with 3,3-diindolylmethane by equilibrium dialysis experiment	Leishmania donovani

Synonyms

Synonyms	Comment	Organism
bi-subunit topoisomerase I	-	Leishmania donovani
DNA topoisomerase	-	Leishmania donovani
LdTOP1LS	-	Leishmania donovani
Topoisomerase I	-	Leishmania donovani

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0012	-	potent inhibitor of Leishmania donovani topoisomerase I	Leishmania donovani	3,3'-diindolylmethane

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Release 2023.1 (January 2023)