5.6.2.1: DNA topoisomerase

This is an abbreviated version!
For detailed information about DNA topoisomerase, go to the full flat file.

Reaction

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining =

Synonyms

ATP-independent type I topoisomerase, AtTop1, bcTopo I, bcTopo IIIbeta, bi-subunit topoisomerase I, Bi-subunit topoisomerase IB, Ca-Top1, CrTop1, Dam DNA topoisomerase III, Deoxiribonucleate topoisomerase, Deoxiribonucleic topoisomerase, DNA Top IB, DNA topo I, DNA topoisomerase, DNA topoisomerase 1, DNA topoisomerase I, DNA topoisomerase I alpha, DNA topoisomerase I-B, DNA topoisomerase IB, DNA topoisomerase III, DNA topoisomerase IIIalpha, DNA topoisomerase type I, EC 5.99.1.2, EcTOP, ETOP, HssTopoI, HsTop1, hTop1p, hTopI, hTopo, htopo I, hTopo IIIalpha, hTopoI, human DNA topoisomerase I, human Top1, human topo I, human topoisomerase I, Isomerase, deoxiribonucleate topo-, Late protein H6, LdTop1, LdTOP1L, LdTOP1LS, LdTOP1S, LdTopIIIbeta, MimiTopIB, MsTopA, MtbTopA, mTopoI, MtTOP1, MttopoI, Nicking-closing enzyme, OjTop1, OlTop1, omega-Protein, OpTop1, PfTopI, PfTopoI, Poxviridae topoisomerase IB, Relaxing enzyme, Reverse gyrase, SiRe_1176, SsTop3, Swivelase, top I, Top1, TOP1alpha, TOP1mt, Top1p, TopA, TopI, TopIB, topo 1, Topo I, topo IB, topo V, topoI, Topoisomerase, topoisomerase 1, topoisomerase 1alpha, Topoisomerase I, topoisomerase I homolog, topoisomerase IB, topoisomerase III, topoisomerase IIIalpha, topoisomerase IIIbeta, topoisomerase IV, topoisomerase V, topoisomerase-I, topoIV, TopR1, TpI, Type I DNA topoisomerase, type I topoisomerase, type IA DNA topoisomerase, type IA topoisomerase, type IB topoisomerase, type IB topoisomerase V, Untwisting enzyme, Vaccinia DNA topoisomerase I, variola topoisomerase IB, vTopo, yeast DNA topoisomerase I, yeastTop1, YpTOP, YrdD, YTOP

ECTree

     5 Isomerases

         5.6 Isomerases altering macromolecular conformation

             5.6.2 Enzymes altering nucleic acid conformation

                5.6.2.1 DNA topoisomerase

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Results	in table
28	Activating Compound
82460	AA Sequence
43	Application
1	CAS Registry Number
71	Cloned(Commentary)
4	Cofactor
14	Crystallization (Commentary)
10906	Disease/ Diagnostics
3	Expression
58	General Information
2	General Stability
208	IC50 Value
1185	Inhibitors
1	Ki Value [mM]
5	KM Value [mM]
79	Localization
131	Metals/Ions
82	Molecular Weight [Da]
71	Natural Substrates/ Products (Substrates)
475	Organism
56	pH Optimum
9	pH Range
1	pH Stability
3	pI Value
1	Posttranslational Modification
261	Protein Variants
93	Purification (Commentary)
16	Reaction
2	Reaction Type
360	Reference
355	Source Tissue
16	Specific Activity [micromol/min/mg]
7	Storage Stability
347	Substrates and Products (Substrate)
69	Subunits
427	Synonyms
1	Systematic Name
61	Temperature Optimum [°C]
8	Temperature Range [°C]
17	Temperature Stability [°C]
1	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 5.6.2.1 - DNA topoisomerase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

15

Rhodobacter capsulatus

-

180 min, stable

649419

25

Rhodobacter capsulatus

-

180 min, about 80% loss of activity. 60 min, about 50% loss of activity

649419

30 - 55

Vaccinia virus

-

5 min, stable

3805

37

Rhodobacter capsulatus

-

180 min, complete loss of activity. 60 min, about 65% loss of activity

649419

40

Brassica oleracea

-

30 min, inactivation

3806

42

Gallus gallus

-

half-life in absence of VO3-: 5 min, half-life in presence of 0.1 mM VO3-: 60 min

3791

44

Saccharomyces cerevisiae

-

50% loss of activity after 8 min, complete inactivation after 40 min

3793

45

3 entries

55

Rhodobacter capsulatus

-

15 min, complete inactivation

649419

60

Alcaligenes sp.

-

pH 7.5, 10 min, complete inactivation

3799

65

2 entries

75

2 entries

80

Thermotoga maritima

P46799

both wild type and C559A/C561A mutant enzymes remain fully active after 30 min

748151

45

Alcaligenes sp.

-

pH 7.5, 10 min, stable

3799

45

Bos taurus

-

10 min, in absence of DNA, quickly inactivated

3803

45

Rhodobacter capsulatus

-

60 min, 90% loss of activity

649419

65

Geobacillus stearothermophilus

-

30 min, 10 mM Tris-HCl buffer, pH 7.5, 1 mM EDTA, 5 mM 2-mercaptoethanol, 0.5 mM PMSF, 10% glycerol, 150 mM NaCl, protein concentration 1.5 mg/ml, enzyme forms MW 80000, MW 100000 and MW 110000, stable

3798

65

Vaccinia virus

-

partial inactivation

3805

75

Geobacillus stearothermophilus

-

30 min, 10 mM Tris-HCl buffer, pH 7.5, 1 mM EDTA, 5 mM 2-mercaptoethanol, 0.5 mM PMSF, 10% glycerol, 150 mM NaCl, protein concentration 1.5 mg/ml, enzyme forms MW 80000, MW 100000 and MW 110000, 30% loss of activity

3798

75

Vaccinia virus

-

5 min, inactivated

3805