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Literature summary for 5.6.2.1 extracted from
- Purification and characterization of type I DNA topoisomerase from Lentinus edodes (1986), FEMS Microbiol. Lett., 37, 169-172.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Double-stranded DNA | - |
Lentinula edodes |  |
| single-stranded DNA | - |
Lentinula edodes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | effectively substituted for Mg2+ in stimulation | Lentinula edodes | |
| K+ | 100 mM KCl stimulates 8fold | Lentinula edodes | |
| Mg2+ | no requirement, 10fold stimulation by 10 mM | Lentinula edodes | |
| Mn2+ | 25% as effective as Mg2+ in stimulation | Lentinula edodes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71500 | - |
x * 71500, SDS-PAGE | Lentinula edodes |
| 235000 | - |
gel filtration | Lentinula edodes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lentinula edodes | - |
- |
- |
| Lentinula edodes FMC2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lentinula edodes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| supercoiled DNA | relaxation | Lentinula edodes | relaxed closed circular DNA | - |
? | |
| supercoiled DNA | positive and negative supercoiled DNA | Lentinula edodes | relaxed closed circular DNA | - |
? | |
| supercoiled DNA | relaxation | Lentinula edodes FMC2 | relaxed closed circular DNA | - |
? | |
| supercoiled DNA | positive and negative supercoiled DNA | Lentinula edodes FMC2 | relaxed closed circular DNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 71500, SDS-PAGE | Lentinula edodes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | 37 | - |
Lentinula edodes |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | about 80% of maximal activity at 20°C and 45°C | Lentinula edodes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Lentinula edodes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | active in this range | Lentinula edodes |
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