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5.6.2.1: DNA topoisomerase

This is an abbreviated version!
For detailed information about DNA topoisomerase, go to the full flat file.

Word Map on EC 5.6.2.1

Reaction

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining =

Synonyms

ATP-independent type I topoisomerase, AtTop1, bcTopo I, bcTopo IIIbeta, bi-subunit topoisomerase I, Bi-subunit topoisomerase IB, Ca-Top1, CrTop1, Dam DNA topoisomerase III, Deoxiribonucleate topoisomerase, Deoxiribonucleic topoisomerase, DNA Top IB, DNA topo I, DNA topoisomerase, DNA topoisomerase 1, DNA topoisomerase I, DNA topoisomerase I alpha, DNA topoisomerase I-B, DNA topoisomerase IB, DNA topoisomerase III, DNA topoisomerase IIIalpha, DNA topoisomerase type I, EC 5.99.1.2, EcTOP, ETOP, HssTopoI, HsTop1, hTop1p, hTopI, hTopo, htopo I, hTopo IIIalpha, hTopoI, human DNA topoisomerase I, human Top1, human topo I, human topoisomerase I, Isomerase, deoxiribonucleate topo-, Late protein H6, LdTop1, LdTOP1L, LdTOP1LS, LdTOP1S, LdTopIIIbeta, MimiTopIB, MsTopA, MtbTopA, mTopoI, MtTOP1, MttopoI, Nicking-closing enzyme, OjTop1, OlTop1, omega-Protein, OpTop1, PfTopI, PfTopoI, Poxviridae topoisomerase IB, Relaxing enzyme, Reverse gyrase, SiRe_1176, SsTop3, Swivelase, top I, Top1, TOP1alpha, TOP1mt, Top1p, TopA, TopI, TopIB, topo 1, Topo I, topo IB, topo V, topoI, Topoisomerase, topoisomerase 1, topoisomerase 1alpha, Topoisomerase I, topoisomerase I homolog, topoisomerase IB, topoisomerase III, topoisomerase IIIalpha, topoisomerase IIIbeta, topoisomerase IV, topoisomerase V, topoisomerase-I, topoIV, TopR1, TpI, Type I DNA topoisomerase, type I topoisomerase, type IA DNA topoisomerase, type IA topoisomerase, type IB topoisomerase, type IB topoisomerase V, Untwisting enzyme, Vaccinia DNA topoisomerase I, variola topoisomerase IB, vTopo, yeast DNA topoisomerase I, yeastTop1, YpTOP, YrdD, YTOP

ECTree

     5 Isomerases
         5.6 Isomerases altering macromolecular conformation
             5.6.2 Enzymes altering nucleic acid conformation
                5.6.2.1 DNA topoisomerase

Cloned

Cloned on EC 5.6.2.1 - DNA topoisomerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
BL21(alphatopB) cells
BL21(DE3)pLysS
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BPV1 glutathione S-transferase (GST)-E1, BPV1 GST-E1 DBD, BPV1 E2, and the human papillomavirus type 11 (HPV11) GST-E1 truncation mutant (aa 589 to 649) expressed in Escherichia coli BL21(DE3)
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cloned into T7 RNA polymerase-regulated plasmid expression vectors, expression in Escherichia coli
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DNA topoisomerase I activity is detected only when both genes LdTOP1A and LdTOP1B are coexpressed in a yeast expression system, LdTOP1A and LdTOP1B are located on different chromosomes
EKY3 yeast cells
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Escherichia coli BL21 (DE3)
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Escherichia coli competent cells BL21(DE3)pLysS, JM109
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expressed in Escherichia coli BL21(DE3)-RIL
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expressed in Escherichia coli BL21(DE3)pLysS cells
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expressed in Escherichia coli T7 Express Crystal cells
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expressed in Escherichia coli. High-copy-number plasmid expressing YpTOP-D117N can not be maintained in Escherichia coli without a second-site mutation
Q7CIL8
expressed in insect cells
expressed in Pichia pastoris strains KM71H and X33
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expressed in Pichia pastoris strains X33, GS115, SMD1168H and KM71H
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expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae strain EKY3
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expressed in Saccharomyces cerevisiae strain RS190
expression in Escherichia coli
expression in Escherichia coli strain RLM1180/pTI-1
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expression in Escherichia coli under the control of a bacteriophage T7 promoter
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expression in Saccharaomyces cerevisiae top1 null strain RS190
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expression in Saccharomyces cerevisiae strain RS190
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expression mutant cells of Saccharomyces cerevisiae
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expression of FLAG-tagged K681A mutant enzyme in Saccharomyces cerevisiae
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expression of His-tagged TopA in Escherichia coli strain BL21
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21AI
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expression of His-tagged Yersinia pestis topoisomerase I mutants, under the control of the BAD promoter in plasmid pYTOP, in Escherichia coli strain JD5 and purification by nickel affinity chromatography leading to a lethal phenotype of the Escherichia coli cells, overview
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expression of mutant enzymes in Escherichia coli
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expression of mutant enzymes in Saccharomyces cerevisiae
expression of the recombinant ETOP is induced in Escherichia coli BL21AI
expression of the soluble His6-tagged enzyme in Escherichia coli strain BL21 (DE3)
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expression of the tagged enzyme in Escherichia coli strain BL21(DE3) from plasmid pPVN123, cloningi in Escherichia coli strain DH10B
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expression of YrdD protein, the homologue of the topoisomerase I C-terminal region, that contains only iron or zinc by expressing the protein in Escherichia coli cells grown in the M9 minimal media supplemented with either iron or zinc, respectively
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full-length cDNA, expression in Escherichia coli
gene MsTopA, co-expression with gene Ms4585, encoding a ribokinase, in Escherichia coli strain BL21. Interaction analysis using the two-hybrid system
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gene MtbTopA, co-expression with gene Rv2436, encoding a ribokinase of Mycobacterium tuberculosis, in Escherichia coli strain BL21. Interaction analysis using the two-hybrid system
gene Sau-TopoI, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain DH5alpha
gene Top1, DNA and amino acid sequence determination and analysis, promoter sequence analysis, genetic structure, sequence comparisons, expression pattern analysis using MPSS method
gene Top1, recombinant expression in Saccharomyces cerevisiae strain EKY3 from YCpGAL1-e-Top1 single copy plasmid, N-terminally epitope-tagged with sequence DYKDDDY
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gene topA, expression fo His-tagged enzyme under the control of a modified pET28 Plac promoter in Escherichia coli strain BL21
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gene topA, expression in Escherichia coli strain BL21(DE3)
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gene topA, the gene is controlled by four promoters recognized by sigma32 and sigmas in addition to sigma70
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Heterologous co-expression of LdTop1L and LdTop1S in a topoisomerase I deficient Saccharomyces cerevisiae strain (EKY3, MBY3), reconstitutes a fully functional enzyme which can be used for structural studies. Purified fragments of N-terminal truncation are used to transform XL-1Blue Escherichia coli
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heterologously expressed in Escherichia coli exhibits topoisomerase activity
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overexpressed in Escherichia coli
overexpressed in yeast
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overexpression in Escherichia coli BL21(DE3)
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overexpression of wild-type and mutant His-tagged and non-tagged enzymes in Escherichia coli strain BL21(DE3)
plasmid-encoded recombinant MtTOP1 can complement the temperature sensitive topA function of Escherichia coli strain AS17 for growth at 42°C. The relaxation activity of recombinant wild-type MtTOP1 is functional in Escherichia coli. No complementation from the Y342A or G116S MtTOP1 mutants, but by mutant H139C, L170C, Y174C, T142C, and K524C. Overexpression of MtTOP1 mutant G116S leads to accumulation of cleavage complex formed by MtTOP1 in Escherichia coli
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recombinant His-tagged full-length enzyme and enzyme fragments expression in Saccharomyces cerevisiae
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Saccharomyces cerevisiae strains EKY2 (MATa, top1D) and EKY3(MATa, top1D)
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three truncated Topo V polypeptides comprising the NH2-terminal 44000 Da and 16000 Da domains, the 44000 Da, 16000 Da and 18000 Da domains and the C-terminal 34000 Da domain
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transformed into the yeast Saccharomyces cerevisiae top1alpha strain RS190 by the lithium acetate procedure
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two genes encoded in ORFs LmjF28.1780 and LmjF36.3200, DNA and amino acid sequence determination and analysis, sequence comparisons. TopIIIbeta subcloning in Escherichia coli DH5alpha, expression in Escherichia coli strain BL21 and in Saccharomyces cerevisiae strain W5909-3B. Expression of C-terminal-GFP-tagged full length LdTopIII? protein in Leishmania donovani promastigotes. LdTopIIIbeta suppresses the yeast top3DELTA slow-growth phenotype