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Literature summary for 5.6.2.1 extracted from
- Physical and functional interaction between D-ribokinase and topoisomerase I has opposite effects on their respective activity in Mycobacterium smegmatis and Mycobacterium tuberculosis (2011), Arch. Biochem. Biophys., 512, 135-142.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene MsTopA, co-expression with gene Ms4585, encoding a ribokinase, in Escherichia coli strain BL21. Interaction analysis using the two-hybrid system | Mycolicibacterium smegmatis |
| gene MtbTopA, co-expression with gene Rv2436, encoding a ribokinase of Mycobacterium tuberculosis, in Escherichia coli strain BL21. Interaction analysis using the two-hybrid system | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the His6 tagged-MtbTopA protein is immobilized on an NTA chip. When an increasing amount of GST tagged-Rv2436 protein pass over the chip, they cause a substantial response, overview | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ms4585 | inhibits the function of MsTopA in relaxing supercoiled DNA. The ribokinase, encoded by gene Ms4585, acts as a netaive regulator of TopA activity | Mycolicibacterium smegmatis | |
| Rv2436 | inhibits the TopA activity on supercoiled DNA. The ribokinase, encoded by gene Rv2436, acts as a netaive regulator of TopA activity | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mycolicibacterium smegmatis | MsTopA stimulates the phosphorylating activity of Ms3759 on D-ribose, and physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview | ? | - |
? |  |
| additional information | Mycobacterium tuberculosis | the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview | ? | - |
? | |
| additional information | Mycobacterium tuberculosis H37Rv | the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WG49 | gene MtbTopA or Rv3646 | - |
| Mycobacterium tuberculosis H37Rv | P9WG49 | gene MtbTopA or Rv3646 | - |
| Mycolicibacterium smegmatis | - |
gene MsTopA | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | MsTopA stimulates the phosphorylating activity of Ms3759 on D-ribose, and physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | substrate is supercoiled plasmid DNA | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | substrate is supercoiled plasmid DNA | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. Interaction analysis, overview | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| additional information | substrate is supercoiled plasmid DNA | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TopA | - |
Mycolicibacterium smegmatis |
| TopA | - |
Mycobacterium tuberculosis |
| Topoisomerase I | - |
Mycolicibacterium smegmatis |
| Topoisomerase I | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycolicibacterium smegmatis |
| 37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Mycolicibacterium smegmatis |
| 7.8 | - |
assay at | Mycobacterium tuberculosis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. While the interaction between the two proteins inhibits the ability of TopA to relax supercoiled DNA, it stimulates ribokinase activity as a regulatory strategy for efficient utilization of D-ribose | Mycolicibacterium smegmatis |
| metabolism | the mycobacterial topoisomerase I physically interacts with its ribokinase both in vitro and in vivo with opposite effects on their respective function, overview. While the interaction between the two proteins inhibits the ability of TopA to relax supercoiled DNA, it stimulates ribokinase activity as a regulatory strategy for efficient utilization of D-ribose | Mycobacterium tuberculosis |
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