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Literature summary for 5.6.2.1 extracted from

  • Gao, R.; Zhang, Y.; Dedkova, L.; Choudhury, A.K.; Rahier, N.J.; Hecht, S.M.
    Effects of modification of the active site tyrosine of human DNA topoisomerase I (2006), Biochemistry, 45, 8402-8410.
    View publication on PubMed
Search for more literature for 5.6.2.1

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli competent cells BL21(DE3)pLysS, JM109 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information Topoisomerase I is modified at the active site by incorporation of a number of tyrosine analogues employed 13 structural analogous of tyrosine. SDS-PAGE, in vitro synthesis of human topoisomerase I analogues modified at position 723. Human topoisomerase I-mediated DNA relaxation assay Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
20-thio camptothecin analogue of camptothecin, inhibit a modified human topoisomerase I Homo sapiens
camptothecin anticancer agent camptothecin (CPT) Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining Human topoisomerase I-mediated DNA relaxation reaction studied following modification of the enzyme at the active site tyrosine (position 723) Homo sapiens
a

Synonyms

Synonyms Comment Organism
DNA topoisomerase I
-
 Homo sapiens