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Literature summary for 5.6.2.1 extracted from

  • Hu, Y.; Clower, R.V.; Melendy, T.
    Cellular topoisomerase I modulates origin binding by bovine papillomavirus type 1 E1 (2006), J. Virol., 80, 4363-4371.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
BPV1 glutathione S-transferase (GST)-E1, BPV1 GST-E1 DBD, BPV1 E2, and the human papillomavirus type 11 (HPV11) GST-E1 truncation mutant (aa 589 to 649) expressed in Escherichia coli BL21(DE3) Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Human Topo I purified from extracts of baculovirus-infected Sf9 or Hi-5 cells Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining demonstrated that human Topo I specifically stimulates the origin binding activity of PV E1, apparently through a protein-protein interaction. Topo I stimulates the origin binding activity of papillomavirus protein E1. Topo I stimulation of E1 binding is highly specific, verified by enzyme-linked immunosorbent assays using a nonorigin DNA fragment, Topo I stimulation of E1 DNA binding activity is origin specific. E1 does not affect the DNA binding activity of Topo I. DNA modulates the interaction between E1 and Topo I (ELISA-based protein interaction assays). Topo I and E2 stimulate the origin binding of E1 independently(enzyme-linked immunosorbent assay) Homo sapiens

Synonyms

Synonyms Comment Organism
Topo I
-
Homo sapiens
Topoisomerase I
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
At 37°C, E1 double hexamers are preferentially formed. The addition of ATP/Mg2+ stimulated the binding activity of E1 at 37°C, but not at 25°C. Optimal stimulation of binding of E1 by Topo I to origin DNA occurred at 37°C in the presence of ATP/Mg 2+ Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 37 The effects of ATP/Mg2+ and temperature on the ability of E1 to bind origin DNA in the presence and absence of Topo I is shown. Increasing amounts of E1 were incubated with radiolabeled PV origin DNA at 37°C or 25°C in the presence or absence of ATP/Mg2+ Homo sapiens