Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.1 extracted from

  • Ahmed, W.; Bhat, A.G.; Leelaram, M.N.; Menon, S.; Nagaraja, V.
    Carboxyl terminal domain basic amino acids of mycobacterial topoisomerase I bind DNA to promote strand passage (2013), Nucleic Acids Res., 41, 7462-7471.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of the tagged enzyme in Escherichia coli strain BL21(DE3) from plasmid pPVN123, cloningi in Escherichia coli strain DH10B Mycolicibacterium smegmatis

Protein Variants

Protein Variants Comment Organism
additional information generation of deletion mutants of the enzyme lacking each one of the three basic stretches of the C-terminal domain. The mutants are highly compromised in binding two DNA molecules Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycolicibacterium smegmatis MstopoI carries out the DNA relaxation reaction in a processive fashion ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant tagged enzyme from Escherichia coli strain BL21(DE3) by heparin affinity chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information MstopoI carries out the DNA relaxation reaction in a processive fashion Mycolicibacterium smegmatis ?
-
 ?
additional information DNA cleavage assay using a 32-mer oligonucleotide harboring strong topoI sites to yield a 5'-end-labeled 19-mer cleavage product. For intramolecular religation, 32-mer oligonucleotide harboring strong topoI sites is incubated with wild-type or deletants to yield a noncovalenty held 19-mer and 13-mer covalent protein-DNA adduct Mycolicibacterium smegmatis ?
-
 ?

Synonyms

Synonyms Comment Organism
topoI
-
 Mycolicibacterium smegmatis
Topoisomerase I
-
 Mycolicibacterium smegmatis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Mycolicibacterium smegmatis

General Information

General Information Comment Organism
additional information the C-terminal domain contains three basic stretches that are essential for the DNA relaxation activity. The basic amino acid stretches confer the non-specific DNA-binding property to the C-terminal domain, overview Mycolicibacterium smegmatis