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Literature summary for 5.6.2.1 extracted from
- Mutational studies reveal lysine 352 on the large subunit is indispensable for catalytic activity of bi-subunit topoisomerase I from Leishmania donovani (2009), Mol. Biochem. Parasitol., 165, 57-66.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Leishmania donovani |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K352A | site-directed mutagenesis, the mutant shows highly reduced relaxation activity compared to wild-type enzyme, the cleavage religation equilibrium is shifted towards the cleavage | Leishmania donovani |
| K352R | site-directed mutagenesis, the mutant shows highly reduced relaxation activity compared to wild-type enzyme, the cleavage religation equilibrium is shifted towards the cleavage | Leishmania donovani |
| R314K | site-directed mutagenesis, the mutant shows onyl slightly affected relaxation activity and only a slightly slower rate of cleavage compared to wild-type enzyme | Leishmania donovani |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Leishmania donovani |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | DNA binding and subunit interaction analysis of wild-type and mutant enzymes, overview | Leishmania donovani | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Leishmania donovani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Topoisomerase I | - |
Leishmania donovani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Leishmania donovani |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Leishmania donovani |
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Release 2023.1 (January 2023)
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