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Literature summary for 5.6.2.1 extracted from

  • Sorokin, E.P.; Cheng, B.; Rathi, S.; Aedo, S.J.; Abrenica, M.V.; Tse-Dinh, Y.C.
    Inhibition of Mg2+ binding and DNA religation by bacterial topoisomerase I via introduction of an additional positive charge into the active site region (2008), Nucleic Acids Res., 36, 4788-4796.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Yersinia pestis
expression of the recombinant ETOP is induced in Escherichia coli BL21AI Escherichia coli

Protein Variants

Protein Variants Comment Organism
M320R site-directed mutagenesis Escherichia coli
M326D substitution mutant Yersinia pestis
M326E substitution mutant Yersinia pestis
M326K substitution mutant Yersinia pestis
M326P substitution mutant Yersinia pestis
M326R overexpression of the M326R mutant YTOP resulted in 4 log loss of viability Yersinia pestis
M326S substitution mutant Yersinia pestis
M326T substitution mutant Yersinia pestis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Yersinia pestis
Mg2+
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P06612
-
-
Yersinia pestis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Yersinia pestis topoisomerase I proteins with the C-terminus hexahistidine tag are purified using the ProPur IMAC Midi spin column Yersinia pestis
single-stranded DNA cellulose column Escherichia coli

Synonyms

Synonyms Comment Organism
ETOP
-
Escherichia coli
Topoisomerase I
-
Yersinia pestis
Topoisomerase I
-
Escherichia coli
YTOP
-
Yersinia pestis