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Literature summary for 5.6.2.1 extracted from
- DNA topoisomerase I domain interactions impact enzyme activity and sensitivity to camptothecin (2015), J. Biol. Chem., 290, 12068-12078 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G717D | the mutant enzyme is no more sensitive to camptothecin than the wild type enzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| camptothecin | - |
Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phosphocellulose column chromatography and FLAG affinity column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| negatively supercoiled pHC624 plasmid DNA | - |
Homo sapiens | relaxed pHC624 plasmid DNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA topoisomerase I | - |
Homo sapiens |
| Top1 | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme catalyzes the relaxation of DNA supercoils | Homo sapiens |
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Release 2023.1 (January 2023)
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