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Literature summary for 5.6.2.1 extracted from

  • Badaracco, G.; Plevani, P.; Ruyechan, W.T.; Chang, L.M.S.
    Purification and characterization of yeast topoisomerase I (1983), J. Biol. Chem., 258, 2022-2026.
    View publication on PubMed
Search for more literature for 5.6.2.1

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ no activity in absence of NaCl or at NaCl concentrations greater than 0.3 M, maximal activity at 0.15 M NaCl Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 sucrose density gradient centrifugation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, stable for at least 2 years Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nicked circular DNA ColE1 DNA Saccharomyces cerevisiae Large catenated DNA networks which appear to rearrange to multimeric linear structures upon long incubation time ?
Nicked circular DNA catenation reaction Saccharomyces cerevisiae Large catenated DNA networks which appear to rearrange to multimeric linear structures upon long incubation time ?
supercoiled DNA relaxation Saccharomyces cerevisiae relaxed closed circular DNA enzyme cannot rewind relaxed closed circular DNA ?
supercoiled DNA positive and negative supercoiled DNA Saccharomyces cerevisiae relaxed closed circular DNA enzyme cannot rewind relaxed closed circular DNA ?
supercoiled DNA ColE1 DNA Saccharomyces cerevisiae relaxed closed circular DNA enzyme cannot rewind relaxed closed circular DNA ?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
 50% loss of activity after 8 min, complete inactivation after 40 min Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 most stable at Saccharomyces cerevisiae