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Literature summary for 5.6.2.1 extracted from

  • Tricoli, J.V.; Kowalski, D.
    Topoisomerase I from chicken erythrocytes: purification, characterization, and detection by a deoxyribonucleic acid binding (1983), Biochemistry, 22, 2025-2031.
    View publication on PubMed
Search for more literature for 5.6.2.1

Inhibitors

Inhibitors Comment Organism Structure
NEM
-
 Gallus gallus
single-stranded DNA
-
 Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Gallus gallus 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ optimal activity at 270 mM KCl Gallus gallus
Mg2+ no requirement for Mg2+ Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 sucrose density gradient sedimentation Gallus gallus
100000
-
 1 * 100000, SDS-PAGE Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is unable to resolve topologically knotted circular duplex DNA Gallus gallus ?
-
 ?
supercoiled DNA relaxation Gallus gallus relaxed closed circular DNA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Gallus gallus