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3.1.3.8: 3-phytase

This is an abbreviated version!
For detailed information about 3-phytase, go to the full flat file.

Word Map on EC 3.1.3.8

Reaction

myo-inositol hexakisphosphate
+
H2O
=
1D-myo-inositol 1,2,4,5,6-pentakisphosphate
 +
phosphate

Synonyms

1-phytase, 168phyA, 3'-phytase, 3-phytase, 3-phytase A, 3-phytase B, acid phytase, acidic histidine acid phosphatase, Afp, agpP, AgpP phytase, alkaline beta-propeller phytase, alkaline phytase, Allzyme, Allzyme phytase, AppA, appA phytase, AppA2, AppA2 phytase, AppAS, appA_Gw, Aspergillus niger N25 phytase, ASR1, AtPAP15, beta-propeller phytase, BIFPSEUDO_03792, Blon_0263, BPP, cell-bound phytase, cysteine phytase, dual-domain BPP, experimental phytase SP 1002, HAP, HAP phytase, HAP-phytase, histidine acid phosphatase, histidine acid phosphatase phytase, histidine acid phytase, HvPAPhy_a, LlALP1, LlALP2, microbial phytase, MiPhyA, MOK1 phytase, More, MYCTH_2306203, myo-inositol 1,2,3,4,5,6-hexakisphosphate phosphohydrolase, myo-inositol hexakiphosphate phosphohydrolase, myo-inositol hexakis phosphohydrolase, myo-inositol hexakisphosphate 3-phosphohydrolase, myo-inositol hexakisphosphate hydrolase, myo-inositol hexakisphosphate phosphohydrolase, myo-inositol hexaphosphate phosphohydrolase, myo-inositol-hexakisphosphate phosphohydrolase, MYO-inositol-hexaphosphate 3-phosphohydrolase, myo-inositol-hexaphosphate phosphohydrolase, myo-inositolhexakisphosphate phosphohydrolase, Natuphos, neutral phytase, NLP_0515, PAP, PAP type I, PAPhy, PAPhys, PAPhy_a1.1, pGF11 phytase, pGP209 phytase, pH 2.5 optimum acid phosphatase, Phy, PHY US42, PHY US573, phy168, PhyA, PhyA phytase, PhyA115, PhyA2, PhyB, PhyB49, PhyC, PhyCm, PhyH, PhyH49, PhyK, phyL, PhyP, phyS, phytase, phytase A, phytase C, phytate 1-phosphatase, Phytate 3-phosphatase, phytate 6-phosphatase, PhyTX52, PJ3 phytase, PPHY, protease-resistant phytase, PSphy, purple acid phosphatase, RMPhy1, RO1, RO2, Ronozyme P, RPHY1, ruminant microbial phytase, St-Phy, StPhy, tfphyA, YeAPPA, YkAPPA, YmPh

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.8 3-phytase

Purification

Purification on EC 3.1.3.8 - 3-phytase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a 1.5fold higher enzyme yield in the presence of sucrose in both submerged and solid-state fermentations, while peptone is found to be a favorable nitrogen source for submerged fermentations. NaH2PO4 favors 34% higher enzyme yield than the control, which is followed by 19% higher activity in KH2PO4 in solid-state fermentation at 0.015% w/v. The addition of Tween-20 in submerged fermentations shows a maximum yield of 12.6 U/ml while, SDS suppresses the growth of the fungus. None of the surfactants favors the enzyme yield in solid-state fermentation. CaCl2 is extensively efficient in stimulating more than 55% higher phytase production in submerged fermentation at 0.01% v/v. In solid-state fermentation, none of the metal salts stimulates phytase production
-
ammonium sulfate fractionation, Sephadex G-75 gel filtration, and DEAE Sepharose Fast Flow column chromatography
-
DEAE cation exchange chromatography and Sephadex100 gel filtration
-
HiPrep Phenyl FF column chromatography
-
Mono S column chromatography and Superose 12 HR 10/30 gel filtration
-
native enzyme 10.1fold by cationic exchange chromatography, anionic exchange chromatography, and chromatofocusing
-
native enzyme 14.22fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, to homogeneity
-
native enzyme 24fold by ethanol precipitation, anion exchange chromatography, and gel filtration
-
native enzyme 35.87fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography, lyophilization, and gel filtration
native enzyme 4.18fold by anion and cation exchange chromatography
-
native enzyme 71.5fold by anion exchange chromatography, cation exchange chromatography, and gel filtration
-
native enzyme 9.53fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
native enzyme by dialysis, anion exchange chromatography, and gel filtration, recombinant His6-tagged enzyme lacking the signal peptide from Escherichia coli by nickel affinity chromatography. Native extracellular enzyme 528fold by dialysis, anion and cation exchange chromatography, and gel filtration
native enzyme from cell culture medium 4.2fold by ammonium sulfate frcationation and dialysis
native enzyme partially by ammonium sulfate fractionation and dialysis
-
native enzyme PhyA 427fold from strain PJ3 by ammonium sulfate fractionation, dialysis, gel filtration, and cation exchange chromatography, and again dialysis, to homogeneity
native extracellular enzyme 10.68fold by ammonium sulfate fractionation, cation exchange chromatography, and gel filtration to homogeneity
native extracellular enzyme 15.2fold by ammonium sulfate fractionation, anion and cation exchange chromatography, and gel filtration, to homogeneity
-
native extracellular enzyme 20.84fold from cell culture supernatant by ethanol precipitation, gel filtration, and anion exchange chromatography
-
native extracellular enzyme 6fold from cell culture medium of strain US573 by heat treatment and ammonium sulfate fractionation
native extracellular enzyme from culture medium 486.41fold by ammonium sulfate fractionation, cation and anion exchange chromatography, and dialysis
native extracellular enzyme from culture medium 5.51fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography, and gel filtration, to near homogeneity
-
Ni-NTA superflow resin column chromatography and HiLoad 16/10 phenyl Sepharose column chromatography
-
nickel-nitrilotriacetic acid metal affinity chromatography, DEAE cation-exchange chromatography, and Sephadex-100 gel filtration
-
partial
partial purification of native enzyme by ammonium sulfate fractionation
Q-DEAE-Sepharose column chromatography
recombinant enzyme
recombinant enzyme 3.3fold from culture supernatant of Pichia pastoris strain GS115 by ammonium sulfate fractionation and gel filtration
recombinant enzyme from Escherichia coli strain BL21(DE3)
KM873028
recombinant enzyme from Pichia pastoris strain X-33 by ultrafiltration, anion exchange chromatography, dialysis, and gel filtration
recombinant extracellular enzyme from Pichia pastoris strain X33 cell culture medium by lyophilization, anion exchange chromatography, and gel filtration, to homogeneity
recombinant extracellular phytase from Pichia pastoris strain X-33 culture medium by lyophilization, dialysis, and anion exchange chromatography
-
recombinant His-tagged enzyme domains PhyH, PhyH-DI, and PhyH-DII from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography followed by two steps of gel filtration to 95% purity
-
recombinant His-tagged full-length enzyme and its N- and C-terminal domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged phytase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography
recombinant His-tagged wild-type PhyK and of mutant H25A PhyK from Escherichia coli strain C41 (DE3) by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3)
recombinant His6-tagged phytase PhyB49 from Escherichia coli strain BL21(DE3)
recombinant His6-tagged phytase PhyH49 from Escherichia coli strain BL21(DE3)
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant isozyme LlALP2-Ala, with the c-myc and (His)6 tags at the C-terminus, from Pichia pastoris strains X-33 and KM71H by cobalt, not nickel, affinity chromatography
recombinant mutant enzymes from Kluyveromyces lactis strain GG799 culture supernatant by lyophilization, anion exchange chromatography, and hydrophobic interaction chromatography
recombinant N-terminally His6-tagged wild-type and mutant enzymes by nickel affinity chromatography
-
recombinant nonglycosylated His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant glycosylated nontagged enzyme from Pichia pastoris strain GS115(his4) by cation exchange chromatography
recombinant protein
recombinant protein, from inclusion bodies
recombinant refolded His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant refolded His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis to homogeneity
recombinant thioredoxin-His-tagged enzyme and His-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant wild-type and mutant enzymes from Kluyveromyces lactis strain GG799 culture supernatant by lyophilization, anion exchange chromatography, and hydrophobic interaction chromatography
recombinant wild-type and mutant enzymes from Pichia pastoris strain GS115 by gel filtration to homogeneity
recombinant wild-type enzyme mutant enzymes from Pichia pastoris strain X33 by ammonium sulfate fractionation, and two different steps of ion exchange chromatography
secreted recombinant wild-type and mutant enzymes from Pichia pastoris strain GS115 culture supernatant
Sephadex G-100 gel filtration and DEAE-Sepharose CL-6B anion exchange column chromatography
-
soluble screted recombinant enzyme from Pichia pastoris culture supernatant by gel filtration
soluble screted recombinant isozyme from Pichia pastoris culture supernatant by gel filtration
the enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
-
the native enzyme is purified to near homogeneity by acetone precipitation and anion exchange chromatography
three phytases LP11, LP12 and LP2
-
two native isozymes RO1 and RO2, 75fold and 46fold, respectively, by acetone fractionation, cation exchange chromatography, gel filtration, and again cation exchange chromatography followed by anion exchange chromatography to homogeneity
-
ultracentrifugation and HiPrep Phenyl FF column chromatography