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Literature summary for 3.1.3.8 extracted from

  • Zhang, R.; Yang, P.; Huang, H.; Yuan, T.; Shi, P.; Meng, K.; Yao, B.
    Molecular and biochemical characterization of a new alkaline beta-propeller phytase from the insect symbiotic bacterium Janthinobacterium sp. TN115 (2011), Appl. Microbiol. Biotechnol., 92, 317-325.
    View publication on PubMed
Search for more literature for 3.1.3.8

Application

Application Comment Organism
additional information PhyA115 is a beta-propeller phytase that has application potential in aquaculture feed Janthinobacterium sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene phyA115, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) Janthinobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, required, optimal at 1.5-2.5 mM, activity profile, overview Janthinobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 x * 64000, SDS-PAGE Janthinobacterium sp.

Organism

Organism UniProt Comment Textmining
Janthinobacterium sp. G0XZM1 symbiotic bacterial strain isolated from the gut contents of Batocera horsfieldi larvae, gene phyA115
-
Janthinobacterium sp. TN115, ACCC 5613 G0XZM1 symbiotic bacterial strain isolated from the gut contents of Batocera horsfieldi larvae, gene phyA115
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) Janthinobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no activity of purified recombinant domain N Janthinobacterium sp.
35
-
 purified recombinant domain C towards phytate, pH 8.5, 45°C Janthinobacterium sp.

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Janthinobacterium sp.
More the enzyme contains an N-terminal incomplete domain, residues 29-297, i.e. domain N, and a typical beta-propeller phytase domain at the C terminus Janthinobacterium sp.

Synonyms

Synonyms Comment Organism
alkaline beta-propeller phytase
-
 Janthinobacterium sp.
PhyA115
-
 Janthinobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 recombinant enzyme Janthinobacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55 more than 40% of the maximal activity at temperatures 25-55°C Janthinobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 purified recombinant enzyme, PhyA115 is unstable without Ca2+, losing about 90% of the activity after incubation at 55°C for 4 min. At high concentrations of Ca2+ of 5-10 mM, PhyA115 retains more than 70% of the activity at 55°C for 30 min. Profiles, overview Janthinobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 recombinant enzyme Janthinobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9 high activity within this range, profile overview Janthinobacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10.5 purified recombinant enzyme, stable, profile overview Janthinobacterium sp.