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Literature summary for 3.1.3.8 extracted from

  • Wang, Y.; Gao, X.; Su, Q.; Wu, W.; An, L.
    Cloning, expression, and enzyme characterization of an acid heat-stable phytase from Aspergillus fumigatus WY-2 (2007), Curr. Microbiol., 55, 65-70.
    View publication on PubMed
Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Aspergillus fumigatus

General Stability

General Stability Organism
incubated with pepsin at 37°C for 2 h at the ratio (pepsin/phytase, wt/wt) of 0.1, the enzyme still retains 90.1% residual activity Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, inhibitive effect Aspergillus fumigatus
Cu2+ 1 mM, inhibitive effect Aspergillus fumigatus
Fe3+ 1 mM, inhibitive effect Aspergillus fumigatus
Zn2+ 1 mM, inhibitive effect Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.114
-
 myo-inositol hexakisphosphate in 200 mM sodium acetate buffer (pH 5.5), at 37°C Aspergillus fumigatus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, slight stimulatory effect Aspergillus fumigatus
Co2+ 1 mM, slight stimulatory effect Aspergillus fumigatus
K+ 1 mM, slight stimulatory effect Aspergillus fumigatus
Mg2+ 1 mM, slight stimulatory effect Aspergillus fumigatus
Mn2+ 1 mM, slight stimulatory effect Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
 SDS-PAGE Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q5XNQ9 3-phytase A precursor
-
Aspergillus fumigatus WY-2 Q5XNQ9 3-phytase A precursor
-

Purification (Commentary)

Purification (Comment) Organism
Q-DEAE-Sepharose column chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus WY-2 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
myo-inositol hexakiphosphate phosphohydrolase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 high ability to resist heat inactivation, after being exposed to 90°C for 15 min, the recombinant enzyme in crude and purified form retains 42% and 43.7% of the initial enzyme activity, respectively Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
102
-
 myo-inositol hexakisphosphate
-
 Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Aspergillus fumigatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
 very stable at the low pH, losing no activity when incubated at pH 2.0 for 4 h Aspergillus fumigatus
7
-
 displays a sharp decline in the activity as the pH moves toward the neutral range, and maintains negligible activity beyond pH 7.0 Aspergillus fumigatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus fumigatus theoretical value
-
 5.95