Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | 2.5 mM, 70% inhibition | Corylus avellana | |
F- | 1 mM, 49% inhibition. 2.5 mM, 61% inhibition | Corylus avellana | |
Fe3+ | 2.5 mM, 68% inhibition | Corylus avellana | |
MgCl2 | 2.5 mM, 20% inhibition | Corylus avellana | |
MnCl2 | 2.5 mM, 17% inhibition | Corylus avellana | |
Na2MoO4 | 2.5 mM, 87% inhibition | Corylus avellana | |
Na3VO4 | 0.1 m, 79% inhibition. 2.5 mM, 90% inhibition | Corylus avellana | |
ZnSO4 | 2.5 mM, 59% inhibition | Corylus avellana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
myo-inositol hexakisphosphate | pH 5.0 | Corylus avellana | |
0.55 | - |
5'-ATP | - |
Corylus avellana | |
1.1 | - |
D-fructose 1,6-diphosphate | - |
Corylus avellana | |
1.2 | - |
D-ribose 5-phosphate | - |
Corylus avellana | |
1.6 | - |
1-naphthyl phosphate | - |
Corylus avellana | |
2.2 | - |
4-nitrophenyl phosphate | pH 5.6 | Corylus avellana | |
2.3 | - |
D-glucose 6-phosphate | - |
Corylus avellana | |
2.4 | - |
phosphate | - |
Corylus avellana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
1 * 71000, SDS-PAGE | Corylus avellana |
72000 | - |
gel filtration | Corylus avellana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corylus avellana | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Corylus avellana |
Purification (Comment) | Organism |
---|---|
- |
Corylus avellana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | of dormant seed | Corylus avellana | - |
seed | dormant | Corylus avellana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.56 | - |
- |
Corylus avellana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | 130% of the activity with myo-inositol hexakisphosphate | Corylus avellana | 1-naphthol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | 101% of the activity with myo-inositol hexakisphosphate | Corylus avellana | 4-nitrophenol + phosphate | - |
? | |
5'-ATP + H2O | 250% of the activity with myo-inositol hexakisphosphate | Corylus avellana | ? | - |
? | |
D-fructose 1,6-diphosphate + H2O | 146% of the activity with myo-inositol hexakisphosphate | Corylus avellana | ? | - |
? | |
D-glucose 6-phosphate + H2O | 78.5% of the activity with myo-inositol hexakisphosphate | Corylus avellana | D-glucose + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | 50% of the activity with myo-inositol hexakisphosphate | Corylus avellana | D-ribose + phosphate | - |
? | |
diphosphate + H2O | 438.5% of the activity with myo-inositol hexakisphosphate | Corylus avellana | 2 phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | step-wise hydrolysis of myo-inositol hexakisphosphate | Corylus avellana | ? + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 71000, SDS-PAGE | Corylus avellana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Corylus avellana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6.5 | 50% of maximal activity at pH 3.5 and pH 6.5 | Corylus avellana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.205 | - |
F- | - |
Corylus avellana |