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3.1.3.8: 3-phytase

This is an abbreviated version!
For detailed information about 3-phytase, go to the full flat file.

Word Map on EC 3.1.3.8

Reaction

myo-inositol hexakisphosphate
+
H2O
=
1D-myo-inositol 1,2,4,5,6-pentakisphosphate
 +
phosphate

Synonyms

1-phytase, 168phyA, 3'-phytase, 3-phytase, 3-phytase A, 3-phytase B, acid phytase, acidic histidine acid phosphatase, Afp, agpP, AgpP phytase, alkaline beta-propeller phytase, alkaline phytase, Allzyme, Allzyme phytase, AppA, appA phytase, AppA2, AppA2 phytase, AppAS, appA_Gw, Aspergillus niger N25 phytase, ASR1, AtPAP15, beta-propeller phytase, BIFPSEUDO_03792, Blon_0263, BPP, cell-bound phytase, cysteine phytase, dual-domain BPP, experimental phytase SP 1002, HAP, HAP phytase, HAP-phytase, histidine acid phosphatase, histidine acid phosphatase phytase, histidine acid phytase, HvPAPhy_a, LlALP1, LlALP2, microbial phytase, MiPhyA, MOK1 phytase, More, MYCTH_2306203, myo-inositol 1,2,3,4,5,6-hexakisphosphate phosphohydrolase, myo-inositol hexakiphosphate phosphohydrolase, myo-inositol hexakis phosphohydrolase, myo-inositol hexakisphosphate 3-phosphohydrolase, myo-inositol hexakisphosphate hydrolase, myo-inositol hexakisphosphate phosphohydrolase, myo-inositol hexaphosphate phosphohydrolase, myo-inositol-hexakisphosphate phosphohydrolase, MYO-inositol-hexaphosphate 3-phosphohydrolase, myo-inositol-hexaphosphate phosphohydrolase, myo-inositolhexakisphosphate phosphohydrolase, Natuphos, neutral phytase, NLP_0515, PAP, PAP type I, PAPhy, PAPhys, PAPhy_a1.1, pGF11 phytase, pGP209 phytase, pH 2.5 optimum acid phosphatase, Phy, PHY US42, PHY US573, phy168, PhyA, PhyA phytase, PhyA115, PhyA2, PhyB, PhyB49, PhyC, PhyCm, PhyH, PhyH49, PhyK, phyL, PhyP, phyS, phytase, phytase A, phytase C, phytate 1-phosphatase, Phytate 3-phosphatase, phytate 6-phosphatase, PhyTX52, PJ3 phytase, PPHY, protease-resistant phytase, PSphy, purple acid phosphatase, RMPhy1, RO1, RO2, Ronozyme P, RPHY1, ruminant microbial phytase, St-Phy, StPhy, tfphyA, YeAPPA, YkAPPA, YmPh

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.8 3-phytase

pH Range

pH Range on EC 3.1.3.8 - 3-phytase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1 - 10
activity range, profile overview
1 - 4.5
-
activity range, profile overview
1 - 5
-
activity range of wild-type and mutant enzyme, profile overview
1 - 8
-
pH profile, overview
1.5 - 3
-
pH 1.5: about 50% of maximal activity, pH 3.0: about 55% of maximal activity. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
1.5 - 6.5
activity range, enzyme mutant R79G, profile, overview
1.8 - 6
-
pH 1.8: about 45% of maximal activity, pH 6.0: about 45% of maximal activity
2 - 12
-
pH 2.0: about 45% of maximal activity, 12.0: about 25% of maximal activity
2 - 5.5
2 - 5.7
-
pH 2.0: about 10% of maximal activity, pH 5.7: about 40% of maximal activity, hydrolysis of myo-inositol hexakisphosphate
2 - 6
2 - 7.5
2 - 9
2.5
wild-type enzyme exhibits high activity at, bi-peak profile
2.5 - 3.5
-
immobilized enzyme
2.5 - 5
2.5 - 5.5
2.5 - 6
-
pH 2.5: about 35% of maximal activity, pH 6.0: about 25% of maximal activity, recombinant enzyme
2.5 - 8
activity range, profile overview, narrow optimum, over 50% activity only at pH 4.0-5.0
2.5 - 8.5
activity range, profile overview, over 50% of activity at pH 3.5-6.5
3 - 3.5
-
free enzyme
3 - 5.5
3 - 6
KM873028
activity range, mesophilic phytase, profile overview
3 - 6.5
activity range, profile overview
3 - 7
3 - 8
3 - 9
3.2 - 5.4
over 70% of the maximum activity within this pH range
3.5 - 5.5
3.5 - 6
3.5 - 6.5
-
50% of maximal activity at pH 3.5 and pH 6.5
3.5 - 7
3.5 - 9
pH profiles of wild-type and mutant enzymes, no activity of mutant enzymes above pH 7.5, overview
3.5 - 9.5
-
activity range, profile overview
3.8 - 5.8
-
pH 3.8: about 55% of maximal activity, pH 5.8: about 70% of maximal activity
4 - 10
-
activity range, pH profile, overview
4 - 5
-
pH 3.0: nearly no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, reaction with 4-nitrophenyl phosphate
4 - 6
4 - 7
4 - 7.5
-
pH 4.0: about 45% of maximal activity, pH 7.5: about 45% of maximal activitry, Q27T mutant enzyme, phytate as substrate
4 - 8
-
pH 4.0: about 50% of maximal activity, pH 8.0: about 25% of maximal activity
4.2 - 6.5
-
pH 4.2: about 80% of maximal activity, pH 6.5: about 70% of maximal activity
4.5 - 5.5
4.5 - 6.5
-
pH 4.5: about 40% of maximal activity, pH 6.5: about 70% of maximal activity, hydrolysis of myo-inositol hexakisphosphate
4.5 - 7
4.5 - 8
-
pH 4.5: about 55% of maximal activity, pH 8.5: about 25% of maximal activity
4.5 - 8.5
5 - 10
-
pH 5.0: about 50% of maximal activity, pH 10.0: about 80% of maximal activity, presence of 10 mM CaCl2
5 - 7
-
pH 5.0: about 90% of maximal activity, pH 7.0: about 50% of maximal activity, at 37°C
5 - 7.5
-
pH 5.0: about 70% of maximal activity, pH 7.5: about 50% of maximal activity
5.5
wild-type enzyme exhibits high activity at, bi-peak profile
5.5 - 7.5
-
pH 5.5: about 35% of maximal activity, pH 7.5: about 95% of maximal activity
5.6 - 7.4
the activity of the wild-tyype enzyme YmPhWT decreases drastically by 14fold from pH 5.6 to pH 7.4
6 - 8
6 - 9
6.3 - 8
-
60-73% of maximal activity within this range
additional information